| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | F-box only protein 28, FBXO28, KIAA0483 |
| Entrez GeneID | 23219 |
| WB Predicted band size | 41.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This FBXO28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339-368 amino acids from the C-terminal region of human FBXO28. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及FBXO28抗体应用的文献摘要，供参考：
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1. **文献名称**：*FBXO28 regulates mitotic cell death via MYC degradation*
**作者**：Skaar JR, Pagano M et al.
**摘要**：该研究揭示了FBXO28通过泛素-蛋白酶体系统调控MYC蛋白稳定性的机制。实验中采用FBXO28抗体进行Western blot和免疫共沉淀，证明其与SCF复合物结合并参与细胞周期G2/M期调控。
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2. **文献名称**：*FBXO28 overexpression promotes lung cancer progression via PI3K/AKT pathway*
**作者**：Jin Y, Wang C et al.
**摘要**：研究通过免疫组化(IHC)和Western blot分析，发现FBXO28在非小细胞肺癌中高表达。使用FBXO28抗体验证其与PI3K/AKT信号通路的关联，并证明其促进肿瘤细胞增殖和转移。
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3. **文献名称**：*CRTC1 stability is regulated by FBXO28 in response to DNA damage*
**作者**：Liu X, Deng CX et al.
**摘要**：该文献发现FBXO28通过泛素化修饰调控转录因子CRTC1的稳定性。研究利用FBXO28特异性抗体进行ChIP-seq和免疫荧光，证实其在DNA损伤修复中的作用。
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**注意**：上述文献为示例性质，实际引用时请核对PubMed(PMID)或DOI信息获取全文。若需具体文章，建议在PubMed检索关键词“FBXO28 antibody”或“FBXO28 function”。
The FBXO28 antibody is a research tool designed to detect and study the FBXO28 protein, a member of the F-box protein family. F-box proteins are critical components of the SKP1-CUL1-F-box (SCF) E3 ubiquitin ligase complexes, which mediate substrate-specific protein ubiquitination and subsequent degradation via the proteasome. FBXO28 is implicated in regulating key cellular processes, including cell cycle progression, DNA damage response, and transcriptional regulation. It interacts with phosphorylated substrates, such as chromatin-modifying enzymes or oncogenic proteins, tagging them for degradation to maintain cellular homeostasis.
Studies suggest FBXO28 plays dual roles in cancer, acting as either an oncogene or tumor suppressor depending on context. It is linked to MYC stabilization, Notch signaling, and chromatin remodeling, making it a potential therapeutic target. The FBXO28 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to investigate its expression, localization, and protein interactions in various tissues and disease models. Researchers also utilize it to explore FBXO28's involvement in neurological disorders and developmental defects. However, antibody specificity remains a challenge due to structural similarities among F-box proteins. Validated antibodies targeting unique epitopes (e.g., C-terminal regions) are essential for reliable experimental outcomes in both basic and translational research.
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