| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ribosomal protein S6 kinase alpha-4, S6K-alpha-4, 90 kDa ribosomal protein S6 kinase 4, Nuclear mitogen- and stress-activated protein kinase 2, Ribosomal protein kinase B, RSKB, RPS6KA4, MSK2 |
| Entrez GeneID | 8986 |
| WB Predicted band size | 85.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RPS6KA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-508 amino acids from the Central region of human RPS6KA4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RPS6KA4抗体的3篇参考文献(基于公开数据模拟，非真实文献)：
1. **"RPS6KA4 regulates tumor metastasis via MAPK/ERK signaling in colorectal cancer"**
- **作者**: Zhang L, et al.
- **摘要**: 研究通过Western blot和免疫组化分析，利用RPS6KA4抗体证实其在结直肠癌组织中高表达，并揭示其通过调控MAPK/ERK通路促进肿瘤转移的机制。
2. **"Functional characterization of RPS6KA4 in neuronal survival under oxidative stress"**
- **作者**: Kim S, et al.
- **摘要**: 该研究使用RPS6KA4特异性抗体进行免疫荧光染色，发现其在神经元中响应氧化应激激活，并通过磷酸化下游靶蛋白Bcl-2抑制细胞凋亡。
3. **"RPS6KA4 as a biomarker for poor prognosis in triple-negative breast cancer"**
- **作者**: Wang Y, et al.
- **摘要**: 通过免疫印迹和组织芯片分析，验证RPS6KA4抗体特异性，发现其高表达与三阴性乳腺癌患者生存率显著相关，提示其作为预后标志物的潜力。
(注：以上为示例性内容，实际文献需通过PubMed或Google Scholar等平台检索确认。)
The RPS6KA4 antibody targets the ribosomal protein S6 kinase A4 (RPS6KA4), a serine/threonine kinase belonging to the RSK (p90 ribosomal S6 kinase) family. Encoded by the *RPS6KA4* gene, this enzyme is also known as MSK2 (mitogen- and stress-activated protein kinase 2). RPS6KA4 is activated via phosphorylation by the ERK/p38 MAPK pathways in response to extracellular stimuli, such as growth factors, hormones, or cellular stress. It regulates diverse cellular processes, including chromatin remodeling, gene transcription, and cell survival, by phosphorylating substrates like CREB, histone H3. and NF-κB. Its activity is linked to inflammation, apoptosis, and neuronal signaling.
RPS6KA4 antibodies are essential tools for studying the protein's expression, localization, and activation in tissues or cultured cells. They are widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Research highlights its role in cancer, neurodegenerative diseases, and immune responses, where dysregulated RPS6KA4 signaling may contribute to pathogenesis. For instance, aberrant RPS6KA4 activity has been implicated in tumor progression and neuroinflammation. When using these antibodies, specificity validation (e.g., knockout controls) and optimal experimental conditions (e.g., sample preparation, blocking buffers) are critical to ensure reliable results. Studies leveraging RPS6KA4 antibodies continue to uncover its therapeutic potential in targeting kinase-driven diseases.
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