Rabbit Polyclonal DNAJB12(N-term) Antibody

The DNAJB12(N-term) antibody is a research tool designed to target the N-terminal region of the DNAJB12 protein, a member of the HSP40 (heat shock protein 40) family. DNAJB12 functions as a co-chaperone, assisting HSP70 proteins in regulating protein folding, quality control, and degradation. It is particularly involved in endoplasmic reticulum (ER)-associated degradation (ERAD), where it helps identify and process misfolded proteins for proteasomal disposal. The N-terminal region of DNAJB12 contains a conserved J-domain critical for interactions with HSP70. enabling ATPase activity and substrate transfer. This antibody is commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study DNAJB12 expression, localization, and interactions under physiological or stress conditions. Research has linked DNAJB12 to diseases such as cancer and neurodegenerative disorders, where protein misfolding and ER stress are hallmarks. By detecting DNAJB12(N-term), scientists aim to elucidate its role in cellular stress responses, disease mechanisms, and potential therapeutic targets. The antibody's specificity for the N-terminal epitope ensures minimal cross-reactivity with other J-domain proteins, making it valuable for mechanistic studies in molecular and cellular biology.