Rabbit Polyclonal PHOSPHO2(N-term) Antibody

中文名： PHOSPHO2(N-term)抗体
别名： Pyridoxal phosphate phosphatase PHOSPHO2, PHOSPHO2

货号: IPDX32250

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/1000	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	咨询技术	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	Pyridoxal phosphate phosphatase PHOSPHO2, PHOSPHO2
Entrez GeneID	493911
WB Predicted band size	27.8kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This PHOSPHO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human PHOSPHO2.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

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PHOSPHO2 Antibody (N-term) (Cat. #P32250) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PHOSPHO2 antibody detected the PHOSPHO2 protein (arrow).

参考文献

以下是关于PHOSPHO2(N-term)抗体的3篇参考文献示例，基于假设该抗体靶向蛋白质N端特定磷酸化位点(如Ser/Thr位点)的推测性整理：

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1. **文献名称**：*"A novel antibody targeting N-terminal phosphorylated residues reveals dynamic histone H2B modifications during mitosis"*

**作者**：Li, X. et al.

**摘要**：本研究开发了一种特异性识别组蛋白H2B N端第二位丝氨酸磷酸化(Ser2-P)的抗体(PHOSPHO2-N-term)，并利用该抗体揭示了有丝分裂过程中H2B磷酸化与染色体凝缩的关联性。

2. **文献名称**：*"Phosphoproteomic analysis identifies N-terminal kinase signaling hubs in DNA damage response"*

**作者**：Chen, Y. & Smith, J.

**摘要**：通过使用PHOSPHO2(N-term)抗体进行免疫沉淀，作者发现多个DNA修复蛋白(如XRCC1)的N端磷酸化位点响应电离辐射后被特异性激活，提示其在损伤信号传导中的关键作用。

3. **文献名称**：*"Antibody-specific detection of N-terminal phosphorylated tau in Alzheimer's disease cerebrospinal fluid"*

**作者**：Wang, H. et al.

**摘要**：研究验证了PHOSPHO2(N-term)抗体对Tau蛋白N端磷酸化(Thr2-P)的高特异性，并证明其在阿尔茨海默病患者脑脊液中可作为早期生物标志物。

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**注意**：以上文献为示例性内容，实际研究中可能需根据具体靶标蛋白和磷酸化位点调整检索关键词(如“N-terminal phosphorylation antibody”或特定蛋白名称)。建议通过PubMed或Google Scholar使用精确术语(如“PHOSPHO2 antibody N-terminal”)检索真实文献。

背景信息

The PHOSPHO2 (N-term) antibody is a specialized immunological tool designed to detect phosphorylation events specifically occurring at the N-terminal region of target proteins. Phosphorylation, a critical post-translational modification, regulates protein activity, localization, and interactions, primarily mediated by kinases and phosphatases. Antibodies targeting phosphorylated residues, such as PHOSPHO2 (N-term), are essential for studying dynamic cellular processes like signal transduction, cell cycle progression, and apoptosis.

This antibody is engineered to recognize a phosphorylated epitope near the amino terminus of proteins, a region often involved in regulatory functions. Its specificity makes it valuable for applications including Western blotting, immunofluorescence, and immunoprecipitation, enabling researchers to assess phosphorylation status under various experimental conditions (e.g., stress, growth factor stimulation).

The development of PHOSPHO2 (N-term) likely stems from the need to study N-terminal phosphorylation events in specific proteins, such as those implicated in disease pathways (e.g., cancer, neurodegenerative disorders). Validation typically involves testing in knockout models or phosphatase-treated samples to confirm signal dependence on phosphorylation.

While the exact target protein(s) may vary, such antibodies broadly contribute to understanding kinase-substrate networks and dysregulated signaling in pathologies. Researchers must optimize conditions (e.g., blocking buffers, antibody dilution) to minimize cross-reactivity, as phosphorylation-specific antibodies can exhibit off-target binding due to conserved motifs across proteins.