| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Potassium channel subfamily K member 6, Inward rectifying potassium channel protein TWIK-2, TWIK-originated similarity sequence, KCNK6, TOSS, TWIK2 |
| Entrez GeneID | 9424 |
| WB Predicted band size | 33.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This KCNK6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-292 amino acids from the C-terminal region of human KCNK6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Characterization of KCNK6 antibody specificity in human tissues"**
- Authors: Smith A, et al.
- 摘要：该研究验证了KCNK6(C-term)抗体在多种人类组织中的特异性，通过Western blot和免疫组化证实其与C末端表位结合，并发现KCNK6在胰腺和神经组织中高表达，提示其潜在生理功能。
2. **"Role of KCNK6 in cancer progression revealed by C-terminal targeting antibodies"**
- Authors: Lee J, et al.
- 摘要：利用KCNK6(C-term)抗体，研究发现KCNK6在乳腺癌细胞中过表达，并通过调节膜电位促进迁移，抗体用于检测临床样本中KCNK6的预后价值。
3. **"KCNK6 channel modulation and antibody-based detection in neuronal disorders"**
- Authors: Garcia-Ruiz C, et al.
- 摘要：通过免疫荧光和流式细胞术，该文献表明KCNK6在小鼠脑部特定神经元群中富集，C-term抗体帮助揭示其与癫痫模型中的电活动异常相关。
4. **"Validation of a novel anti-KCNK6 antibody for functional studies"**
- Authors: Müller T, et al.
- 摘要：研究开发并验证了一种高特异性KCNK6(C-term)抗体，用于共聚焦成像和蛋白质相互作用分析，证明KCNK6与调控蛋白TASK-1的共定位关系。
(注：以上文献为示例性概括，实际引用需根据具体论文调整。)
The KCNK6 (C-term) antibody is designed to target the C-terminal region of the potassium channel subfamily K member 6 (KCNK6), also known as TWIK-2 or TASK-1. KCNK6 is a member of the two-pore domain (K2P) potassium channel family, which plays a critical role in regulating cellular resting membrane potential and excitability. This protein is characterized by its unique structure, featuring two pore-forming domains and four transmembrane segments, enabling background "leak" potassium currents. KCNK6 is expressed in various tissues, including the brain, heart, kidneys, and immune cells, and is implicated in physiological processes such as pH sensing, cell volume regulation, and apoptosis. Dysregulation of KCNK6 has been linked to pathologies like cancer, cardiovascular diseases, and neurological disorders.
The KCNK6 (C-term) antibody is commonly used in research to study the expression, localization, and functional roles of KCNK6 through techniques like Western blotting, immunohistochemistry, and immunofluorescence. Its specificity for the C-terminal region allows researchers to distinguish KCNK6 from other K2P channels and investigate post-translational modifications or protein-protein interactions critical for channel activity. This antibody has been instrumental in elucidating KCNK6's involvement in disease mechanisms, such as its overexpression in certain cancers promoting cell proliferation or its role in modulating immune responses. Validation data typically include reactivity in relevant cell lines or tissues and comparison with knockdown/knockout controls. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary by supplier, influencing experimental design.
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