| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human OTX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LHX4抗体的3篇参考文献示例，涵盖其在不同研究中的应用及发现：
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1. **文献名称**: *LHX4 regulates pituitary gland development through transcriptional activation of progenitor cell genes*
**作者**: Mullen RD, et al.
**摘要**: 本研究利用针对LHX4中心结构域的多克隆抗体，通过免疫组化和染色质免疫沉淀技术(ChIP)，揭示了LHX4在小鼠垂体前叶祖细胞分化中的关键调控作用，并发现其突变会导致联合垂体激素缺乏症(CPHD)。
2. **文献名称**: *A novel monoclonal antibody targeting the LHX4 protein reveals its expression in human neuroendocrine tumors*
**作者**: Garcia-Belmonte R, et al.
**摘要**: 研究团队开发了一种高特异性小鼠单克隆抗体(克隆号：2C4)，针对LHX4的中心区域，用于检测神经内分泌肿瘤中LHX4的异常表达。通过免疫印迹和免疫荧光证实，该抗体在临床样本中具有高灵敏度和低交叉反应性。
3. **文献名称**: *Developmental regulation of LHX4 in the murine hypothalamus and its role in neuronal differentiation*
**作者**: Kimura S, et al.
**摘要**: 采用兔源LHX4抗体(靶向中心结构域)进行免疫组织化学分析，发现LHX4在小鼠下丘脑发育过程中动态表达，并调控神经元的迁移与分化，为相关先天性神经系统疾病提供了分子机制解释。
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**备注**：上述文献为示例，实际引用时需核实来源及详细信息。若需具体文献，建议通过PubMed或Google Scholar以关键词“LHX4 antibody” + “immunohistochemistry/Western blot”检索近年研究。
The LHX4 (Center) antibody is a specialized tool used to detect the LHX4 protein, a member of the LIM-homeodomain (LIM-HD) transcription factor family. LHX4 plays critical roles in embryonic development, particularly in the formation and differentiation of the pituitary gland, nervous system, and other organs. It regulates gene expression by binding to DNA and interacting with co-factors to control cell fate, proliferation, and survival. Mutations in the LHX4 gene are linked to congenital pituitary hormone deficiencies and structural brain abnormalities, making it a focus in developmental biology and disease research.
The LHX4 (Center) antibody specifically targets a central epitope within the protein, ensuring precise detection in assays like immunohistochemistry (IHC), Western blot (WB), and immunofluorescence (IF). Its design often relies on immunogen sequences corresponding to conserved regions, ensuring cross-reactivity across species such as humans, mice, and rats. Researchers use this antibody to study LHX4's spatial and temporal expression patterns, its role in organogenesis, and its interplay with other transcription factors like PROP1 or POU1F1. Validated for specificity and sensitivity, it aids in exploring molecular mechanisms underlying developmental disorders and potential therapeutic targets. Commercial suppliers typically provide detailed validation data, including applications in tissue sections or cell lysates, to support experimental reproducibility.
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