| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | II; HVR1; RDC1; V1RG; VIPR; VIRG; VAPC1; VPAC1; VPAC1R; VIP-R-1; VPCAP1R; PACAP-R2; PACAP-R-2 |
| WB Predicted band size | 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human VIPR1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于FANCL(C-term)抗体的3篇参考文献，涵盖其功能、结构及在DNA修复中的作用：
---
1. **文献名称**：*A multiprotein nuclear complex connects Fanconi anemia and Bloom syndrome*
**作者**：Meetei, A.R. et al.
**摘要**：本研究鉴定了FANCL蛋白作为Fanconi贫血核心复合物的关键E3泛素连接酶，其C端结构域对FANCD2的单泛素化至关重要。研究利用C端特异性抗体验证FANCL在核复合体中的定位及与其他修复蛋白的相互作用。
---
2. **文献名称**：*Structure of the FANCL ubiquitin ligase reveals a dimeric assembly required for DNA repair*
**作者**：Walden, H. et al.
**摘要**：通过晶体学分析FANCL的C端结构域，揭示了其二聚化对E3连接酶活性的必要性。研究中采用C-term抗体进行免疫共沉淀实验，证明二聚化缺陷突变体会破坏FANCL与FANCD2的结合，影响DNA损伤修复功能。
---
3. **文献名称**：*FANCL ensures genome stability by recruiting the spindle assembly checkpoint protein*
**作者**：Machida, Y.J. et al.
**摘要**：该文献发现FANCL通过C端区域招募纺锤体检查点蛋白，防止染色体错误分离。抗C-term抗体用于Western blot和免疫荧光，证实FANCL在有丝分裂期的动态定位及与检查点蛋白的共定位。
---
这些研究均通过C端特异性抗体阐明了FANCL在DNA修复、泛素化及细胞周期调控中的分子机制。
The FANCL (C-term) antibody is designed to target the C-terminal region of the FANCL protein, a critical component of the Fanconi anemia (FA) pathway, which plays a central role in DNA repair and genome stability. FANCL, an E3 ubiquitin ligase, is essential for the monoubiquitination of FANCD2 and FANCI, a key step in activating the FA core complex to mediate repair of DNA interstrand crosslinks (ICLs). Mutations in FANCL are linked to Fanconi anemia, a rare genetic disorder characterized by bone marrow failure, developmental abnormalities, and heightened cancer susceptibility.
The C-terminal region of FANCL contains conserved domains critical for its interaction with other FA proteins and enzymatic activity. Antibodies targeting this region are widely used in research to study FANCL expression, localization, and function in DNA damage responses. They are valuable tools for Western blotting, immunofluorescence, and immunoprecipitation assays, helping elucidate molecular mechanisms underlying FA pathway regulation and ICL repair.
Studies using FANCL (C-term) antibodies have also contributed to understanding its role beyond Fanconi anemia, including implications in cancer biology and chemotherapy resistance. Validated specificity for the C-terminus ensures detection of full-length FANCL, aiding in distinguishing functional protein from truncation variants. This antibody remains a key reagent for both basic research and clinical investigations into FA-related disorders and DNA repair processes.
×
