| WB | DB: 1/500 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bad |
| Entrez GeneID | 64639 |
| WB Predicted band size | 22.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | This rat BAD Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S97 of rat BAD. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-rat BAD(S97)抗体的假设参考文献示例，供参考格式使用：
1. **标题**：*Phosphorylation of BAD at Serine 97 Modulates Apoptosis in Rat Neuronal Cells*
**作者**：Zhang L, et al.
**摘要**：研究利用Phospho-rat BAD(S97)抗体，证实脑缺血模型中BAD的Ser97磷酸化通过阻断Bcl-2相互作用抑制神经元凋亡，提示其神经保护机制。
2. **标题**：*Akt-Mediated BAD Ser97 Phosphorylation Enhances Survival in Rat Cardiomyocytes*
**作者**：Chen R, et al.
**摘要**：通过该抗体检测到Akt激酶对BAD Ser97的磷酸化调控，发现其通过抑制线粒体凋亡通路改善心肌细胞缺氧损伤。
3. **标题**：*Validation of a Phospho-Specific Antibody for Rat BAD (Ser97) in Hepatic Fibrosis Models*
**作者**：Kim S, et al.
**摘要**：报道了Phospho-rat BAD(S97)抗体的开发与验证，并应用于肝纤维化研究，显示磷酸化水平与肝细胞存活率正相关。
4. **标题**：*BAD Ser97 Phosphorylation in Rat Ovarian Follicle Atresia: Role of ERK Signaling*
**作者**：Martinez P, et al.
**摘要**：使用该抗体揭示ERK通路介导的BAD Ser97磷酸化延缓卵泡闭锁，为生殖细胞凋亡机制提供新见解。
**注**：以上文献为示例，实际引用时需查询真实数据库(如PubMed)并核实信息。若需真实文献，建议补充具体实验背景或联系文献服务。
Phospho-rat BAD(S97) antibody is a specialized tool used to detect the phosphorylated form of the BAD protein (Bcl-2-associated death promoter) at serine residue 97 in rat-derived samples. BAD, a pro-apoptotic member of the Bcl-2 family, regulates programmed cell death by interacting with anti-apoptotic proteins like Bcl-2 and Bcl-xL. Its activity is tightly controlled by phosphorylation, which inactivates its pro-apoptotic function. Phosphorylation at Ser97. along with other residues (e.g., Ser136. Ser155), promotes BAD’s dissociation from anti-apoptotic partners and its sequestration in the cytoplasm via binding to 14-3-3 proteins, thereby enhancing cell survival. This modification is often mediated by survival signaling pathways, such as those activated by growth factors (e.g., IL-3. IGF-1) or kinases like AKT/PKB.
The Phospho-rat BAD(S97) antibody is essential for studying apoptosis regulation in rat models, particularly in contexts like cancer, neurodegeneration, or developmental biology. Researchers use it in techniques such as Western blotting, immunohistochemistry, or immunocytochemistry to assess BAD activation status under experimental conditions (e.g., drug treatments, stress responses). Specificity for the phosphorylated epitope ensures accurate detection of BAD’s inactive state, making it valuable for exploring mechanisms of cell survival/death balance. Cross-reactivity with BAD homologs in other species should be verified for broader applications. This antibody contributes to understanding disease mechanisms and potential therapies targeting apoptosis pathways.
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