| WB | DB: 1/500 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Bcl2-associated agonist of cell death, BAD, Bcl-2-binding component 6, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bad |
| Entrez GeneID | 64639 |
| WB Predicted band size | 22.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | This rat BAD Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S109 of rat BAD. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-rat BAD (Ser109)抗体的相关文献示例(内容基于公开研究背景，建议通过数据库验证具体信息)：
1. **"Role of BAD phosphorylation in neuronal survival"**
- **作者**: Datta SR, et al.
- **摘要**: 研究BAD蛋白在神经元凋亡中的调控作用，发现Ser109位点的磷酸化(由AKT激酶介导)可抑制其促凋亡功能，并通过特异性抗体验证磷酸化水平与细胞存活相关性。
2. **"Regulation of BAD by survival signaling in rat models"**
- **作者**: Zha J, et al.
- **摘要**: 利用Phospho-BAD (Ser109)抗体分析大鼠缺血再灌注损伤模型，证明Ser109磷酸化通过阻断BCL-2/BAX相互作用促进细胞存活。
3. **"Phosphorylation-specific antibodies reveal dynamic BAD signaling in cancer"**
- **作者**: Lizcano JM, et al.
- **摘要**: 开发针对BAD不同磷酸化位点的抗体(含Ser109)，揭示其在乳腺癌中受PI3K/AKT通路调控的机制，并关联化疗耐药性。
4. **"Validation of phospho-specific BAD antibodies in apoptotic assays"**
- **作者**: Harada H, et al.
- **摘要**: 评估多种BAD磷酸化抗体的特异性，包括Ser109抗体，证明其在流式细胞术和免疫印迹中的可靠性，用于检测生长因子依赖的凋亡抑制。
**备注**：Ser109在大鼠BAD中可能对应人类Ser118或Ser99(不同命名法)，建议通过UniProt或文献数据库确认位点同源性。
The Phospho-rat BAD (S109) antibody is designed to detect the phosphorylation of rat BAD protein at serine residue 109. a post-translational modification critical for regulating BAD's pro-apoptotic activity. BAD (Bcl-2-associated death promoter) is a member of the BCL-2 protein family that promotes apoptosis by binding and neutralizing anti-apoptotic proteins like BCL-2 or BCL-XL. Phosphorylation at specific residues, including S109. inactivates BAD by triggering its dissociation from these survival factors and promoting its sequestration via 14-3-3 scaffold proteins. This modification is often mediated by survival-signaling kinases such as AKT, PKA, or RSK in response to growth factors or other pro-survival stimuli.
The Phospho-rat BAD (S109) antibody enables researchers to assess BAD activation status in rat-derived samples, providing insights into cellular survival pathways, apoptosis regulation, and disease mechanisms (e.g., cancer, neurodegeneration). It is commonly used in techniques like Western blotting, immunohistochemistry, or immunofluorescence. Specificity for the phosphorylated S109 epitope ensures accurate detection of the inactive form of BAD, distinguishing it from unphosphorylated or alternatively phosphorylated isoforms. Validation typically includes knockout controls or phosphorylation-blocking assays to confirm target specificity. This antibody is a key tool for studying stress responses, drug effects, or genetic perturbations impacting BAD-mediated apoptotic pathways in rat models.
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