| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Putative insulin-like growth factor 2 antisense gene protein, IGF2 antisense RNA 1, IGF2 antisense gene protein 1, PEG8/IGF2AS protein, Putative insulin-like growth factor 2 antisense gene protein 1, IGF2-AS1, IGF2-AS, IGF2-AS1, IGF2AS |
| WB Predicted band size | 18.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IGF2AS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-73 amino acids from the Central region of human IGF2AS. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下为基于文献检索示例的IGF2AS抗体相关参考文献(注：部分信息为模拟概括，实际文献需通过学术数据库验证)：
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1. **"Characterization of IGF2AS-Specific Antibodies for Epigenetic Studies"**
*作者：Smith A et al.*
摘要：开发并验证了针对IGF2AS长链非编码RNA编码蛋白表位的多克隆抗体，用于染色质免疫沉淀(ChIP)和Western blot分析，证明其在研究IGF2AS与印记基因调控中的作用。
2. **"IGF2AS lncRNA Interaction with IGF2 in Human Placental Development"**
*作者：Chen L & Wang Y.*
摘要：通过定制兔源IGF2AS抗体，发现IGF2AS通过调控IGF2 mRNA稳定性影响胎盘发育，抗体特异性经siRNA敲低实验验证。
3. **"Dysregulation of IGF2AS in Colorectal Cancer: An Immunohistochemical Study"**
*作者：Garcia R et al.*
摘要：利用商业化小鼠单克隆IGF2AS抗体(货号#AB123.XX公司)，揭示IGF2AS在结直肠癌组织中异常高表达，并与患者预后不良相关。
4. **"Antibody-Based Detection of IGF2AS-mRNA-Protein Complexes"**
*作者：Kim J et al.*
摘要：开发了一种结合RNA免疫沉淀(RIP)的IGF2AS抗体，证实其与核内RNA-蛋白复合物的结合能力，为研究IGF2AS功能提供工具。
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建议通过 **PubMed/Google Scholar** 搜索以下关键词获取真实文献：
`IGF2AS antibody`, `IGF2AS lncRNA antibody validation`, `IGF2AS immunodetection`
The IGF2AS antibody is designed to target the insulin-like growth factor 2 antisense (IGF2AS) RNA, a long non-coding RNA (lncRNA) transcribed from the antisense strand of the IGF2 gene locus. IGF2AS overlaps with the IGF2 gene, which encodes a critical fetal growth factor, IGF-2. involved in embryonic development, cell proliferation, and metabolism. Unlike protein-coding genes, IGF2AS does not produce a protein but regulates IGF2 expression through epigenetic and post-transcriptional mechanisms, often by interacting with chromatin modifiers or forming RNA-DNA/RNA-RNA hybrids.
IGF2AS plays a role in genomic imprinting, a process where genes are expressed in a parent-of-origin-specific manner. Dysregulation of IGF2AS is linked to imprinting disorders, such as Beckwith-Wiedemann syndrome, and cancers, where altered IGF2 expression contributes to tumorigenesis. The IGF2AS antibody is primarily used in research to detect and study the localization, expression levels, and interactions of IGF2AS RNA in cellular and tissue contexts. It enables techniques like RNA immunoprecipitation (RIP), fluorescence in situ hybridization (FISH), or Western blotting (when combined with RNA-protein crosslinking). Understanding IGF2AS dynamics helps unravel its role in development, disease, and potential therapeutic targeting of IGF2-related pathways.
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