| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA; AKT1 |
| Entrez GeneID | 207 |
| clone | 3A3 |
| WB Predicted band size | 56kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey |
| Immunogen | Purified recombinant fragment of human AKT1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CDC42EP2抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **文献名称**：*CDC42EP2 regulates cell polarity and cytoskeletal organization during collective cell migration*
**作者**：Smith A, et al.
**摘要**：本研究利用CDC42EP2特异性抗体，通过免疫荧光和Western blot分析，揭示了CDC42EP2在调控上皮细胞迁移过程中细胞极性和肌动蛋白骨架重塑的作用，表明其通过与CDC42的相互作用影响细胞集体运动。
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2. **文献名称**：*Characterization of CDC42EP2/BORG2 as a binding partner of septin complexes in mammalian cells*
**作者**：Li Y, et al.
**摘要**：通过免疫共沉淀(使用CDC42EP2抗体)和质谱分析，作者发现CDC42EP2与septin蛋白复合物结合，参与细胞分裂过程中胞质分裂的调控，提示其在细胞周期中的潜在功能。
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3. **文献名称**：*CDC42EP2 deficiency disrupts vascular development via dysregulated Rho GTPase signaling*
**作者**：Chen H, et al.
**摘要**：研究利用基因敲除模型和CDC42EP2抗体进行组织染色，证明CDC42EP2缺失导致血管内皮细胞形态异常，影响Rho GTPase信号通路，进而破坏血管生成。
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**备注**：以上文献为示例性质，实际文献需通过PubMed、Google Scholar或抗体供应商(如Abcam、CST)提供的引用列表进一步检索确认。
The CDC42EP2 antibody is a crucial tool for studying the CDC42 effector protein 2 (CDC42EP2), a member of the CDC42EP/BORG family that interacts with the Rho GTPase CDC42. CDC42EP2 regulates cytoskeletal dynamics, cell polarity, and membrane protrusion by binding CDC42 via its conserved CRIB (CDC42/Rac-interactive binding) domain. It facilitates the formation of protein complexes that modulate actin reorganization, impacting cell morphology, migration, and cell-cell adhesion.
Antibodies targeting CDC42EP2 are widely used in research to investigate its expression, localization, and functional roles in cellular processes. These antibodies are typically developed in hosts like rabbits or mice, using immunogens derived from specific regions of the human CDC42EP2 protein. Validated applications include Western blotting, immunofluorescence, and immunoprecipitation, often with verification via knockout/knockdown controls to ensure specificity.
CDC42EP2 has been implicated in pathological conditions, including cancer metastasis, inflammation, and vascular disorders, due to its role in cytoskeletal remodeling. Studies also explore its involvement in developmental processes and immune responses. Researchers rely on CDC42EP2 antibodies to elucidate its interaction networks, post-translational modifications, and downstream signaling pathways. Proper validation and optimization of these antibodies are critical for reproducibility, particularly given the high homology among CDC42EP family members. Their use continues to advance understanding of CDC42-driven cellular mechanisms in health and disease.
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