| WB | 1/1000 | Human, Mouse, zebrafish |
| IF | 咨询技术 | Human, Mouse, zebrafish |
| IHC | 1/100-1/500 | Human, Mouse, zebrafish |
| ICC | 技术咨询 | Human, Mouse, zebrafish |
| FCM | 咨询技术 | Human, Mouse, zebrafish |
| Elisa | 咨询技术 | Human, Mouse, zebrafish |
| Aliases | Dual specificity mitogen-activated protein kinase kinase 4, MAP kinase kinase 4, MAPKK 4, C-JUN N-terminal kinase kinase 1, JNK kinase 1, JNKK 1, JNK-activating kinase 1, MAPK/ERK kinase 4, MEK 4, SAPK/ERK kinase 1, SEK1, Map2k4, Jnkk1, Mek4, Mkk4, Prkmk4, Sek1, Serk1, Skk1 |
| Entrez GeneID | 26398 |
| WB Predicted band size | 44.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, zebrafish |
| Immunogen | This Mouse Map2k4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the C-terminal region of mouse Map2k4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Map2k4抗体的3篇参考文献示例：
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1. **文献名称**：*Targeted Disruption of the Mouse Map2k4 Gene Reveals Essential Roles in Embryonic Development and Stress Response*
**作者**：Yujiro Tanaka, et al.
**摘要**：本研究通过基因敲除技术构建了Map2k4缺陷小鼠模型，并利用特异性抗体进行Western blot和免疫组化分析。结果显示，Map2k4缺失导致胚胎致死及胎盘发育异常，证实其在应激信号通路(如JNK激活)中的关键作用。
2. **文献名称**：*MAP2K4 Deficiency Alters Tumor Microenvironment and Promotes Hepatocellular Carcinoma Progression*
**作者**：Li Chen, et al.
**摘要**：通过免疫荧光和流式细胞术结合Map2k4抗体，研究发现肝癌模型中Map2k4表达下调与JNK/SAPK通路失调相关，提示其作为肿瘤抑制因子的潜在机制。
3. **文献名称**：*Differential Roles of MAP2K4 in Neuronal Apoptosis and Inflammation*
**作者**：Emily R. Johnson, et al.
**摘要**：使用Map2k4抗体检测小鼠脑组织中的蛋白表达，发现Map2k4通过调控下游激酶活性参与神经元凋亡和炎症反应，为神经退行性疾病研究提供新靶点。
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注：以上文献为示例，实际引用时需根据具体研究内容检索PubMed等数据库获取真实文献(如PMID: 12345678)。若需进一步精准检索，可结合关键词“Map2k4 antibody”、“MEK4 mouse model”等筛选。
The Mouse MAP2K4 (Mitogen-Activated Protein Kinase Kinase 4) antibody is a tool designed to detect and study the MAP2K4 protein, also known as MEK4 or MKK4. in mouse-derived samples. MAP2K4 is a serine/threonine kinase within the MAPK signaling pathway, acting as a critical mediator of cellular responses to stress, cytokines, and growth signals. It phosphorylates and activates downstream kinases such as JNK (c-Jun N-terminal kinase) and p38 MAPK, which regulate processes like apoptosis, differentiation, and inflammation. Dysregulation of MAP2K4 is linked to cancer, immune disorders, and neurodegenerative diseases.
The antibody is commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to investigate MAP2K4 expression, localization, and function in mouse tissues or cell lines. It is often validated for specificity to ensure minimal cross-reactivity with related kinases (e.g., MAP2K1/MEK1). Researchers employ this antibody to explore MAP2K4's role in stress response pathways, tumor suppression (e.g., its frequent inactivation in cancers), or interactions with upstream regulators like MAP3Ks and downstream effectors. Its application aids in understanding disease mechanisms and potential therapeutic targets. Optimal performance typically requires validation under specific experimental conditions, including proper sample preparation and antibody dilution.
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