| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Eukaryotic translation initiation factor 1b, eIF1b, Protein translation factor SUI1 homolog GC20, EIF1B |
| Entrez GeneID | 10289 |
| WB Predicted band size | 12.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This EIF1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-113 amino acids from the C-terminal region of human EIF1B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于EIF1B抗体的假设性参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**：*EIF1B modulates translation initiation fidelity in human cells*
**作者**：Zhang Y, et al.
**摘要**：本研究利用EIF1B特异性抗体，通过免疫共沉淀和Western blot分析，揭示了EIF1B在调控真核翻译起始准确性中的作用，并发现其表达异常与核糖体错读相关。
2. **文献名称**：*Antibody-based profiling of EIF1B expression in colorectal cancer*
**作者**：Smith J, et al.
**摘要**：通过免疫组化技术结合EIF1B多克隆抗体，作者发现EIF1B在结直肠癌组织中显著高表达，提示其可能作为潜在生物标志物或治疗靶点。
3. **文献名称**：*Subcellular localization of EIF1B in neuronal cells*
**作者**：Lee S, Kim M.
**摘要**：利用EIF1B抗体进行免疫荧光染色，证实该蛋白主要定位于神经元胞质中的多核糖体区域，支持其在神经发育相关蛋白翻译中的功能。
4. **文献名称**：*Comparative analysis of commercial EIF1B antibodies for specificity validation*
**作者**：Brown R, et al.
**摘要**：系统性评估了多种市售EIF1B抗体的特异性，通过siRNA敲低实验验证了抗体可靠性，为后续研究提供了选用指南。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词“EIF1B antibody”、“Eukaryotic Initiation Factor 1B”结合研究领域(如癌症、翻译调控)进行精准查询。
The eukaryotic translation initiation factor 1B (EIF1B), also known as eIF2-associated glycoprotein (p67), is a component of the eukaryotic translation initiation complex. It plays a critical role in protein synthesis by regulating the assembly of the 43S pre-initiation complex and ensuring accurate start codon recognition. EIF1B interacts with eIF2. a GTP-binding protein essential for delivering the initiator tRNA to the ribosome, and modulates its activity during the initiation phase. Dysregulation of EIF1B has been linked to cellular stress responses, oncogenesis, and viral infection mechanisms.
EIF1B antibodies are widely used in research to investigate its expression, localization, and function in physiological and pathological contexts. Studies have shown elevated EIF1B levels in certain cancers, including prostate, ovarian, and thyroid cancers, suggesting its potential as a biomarker or therapeutic target. These antibodies enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore EIF1B's role in translation control, cell proliferation, and apoptosis. Additionally, they aid in studying interactions with other initiation factors or viral proteins that hijack host translation machinery. Commercial EIF1B antibodies are typically raised against specific epitopes, ensuring specificity for human, mouse, or rat homologs, and are validated for applications across diverse experimental models.
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