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Rabbit Polyclonal MouseCamk2a Antibody

  • 中文名: Mouse Camk2a抗体
  • 别    名: Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, Camk2a
货号: IPDX31532
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCalcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, Camk2a
Entrez GeneID12322
WB Predicted band size54.1kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityMouse
ImmunogenThis Mouse Camk2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-349 amino acids from the C-terminal region of mouse Camk2a.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是几篇关于小鼠CamKIIα(Camk2a)抗体的参考文献示例(注:以下内容为虚构示例,仅用于演示格式,实际文献请通过PubMed或Google Scholar检索):

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1. **文献名称**: "Calcium/calmodulin-dependent protein kinase II alpha (Camk2a) expression in hippocampal synaptic plasticity"

**作者**: Smith J, et al.

**摘要**: 本研究通过Western blot和免疫荧光技术,使用特异性Camk2a抗体证实其在成年小鼠海马神经元中的高表达,并揭示其在长时程增强(LTP)中的动态变化。

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2. **文献名称**: "Validation of a monoclonal Camk2a antibody for detecting neuronal activity in transgenic mice"

**作者**: Chen L, et al.

**摘要**: 文章验证了一种高特异性Camk2a单克隆抗体在小鼠脑组织中的应用,通过免疫印迹和免疫组化证明其可区分Camk2a与其他亚型(如Camk2b),并用于分析学习行为模型中Camk2a的磷酸化状态。

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3. **文献名称**: "Camk2a knockdown in a mouse model of Alzheimer's disease: Antibody-based quantification and localization"

**作者**: Tanaka R, et al.

**摘要**: 利用Camk2a多克隆抗体对阿尔茨海默病模型小鼠前额叶皮层进行定量分析,发现Camk2a表达水平与突触功能损伤显著相关,抗体特异性通过siRNA敲低实验验证。

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4. **文献名称**: "Phosphorylation-specific Camk2a antibody reveals dynamic changes in memory consolidation"

**作者**: Müller F, et al.

**摘要**: 开发了一种针对Camk2a Thr286磷酸化位点的抗体,应用于小鼠脑切片和原代神经元,证明其在记忆巩固过程中磷酸化Camk2a的时空分布特征。

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如需真实文献,建议在PubMed中检索关键词:**Camk2a antibody mouse**,并筛选涉及Western blot、IHC、或神经元研究的文章。

背景信息

The mouse Camk2a antibody is a crucial tool for studying the calcium/calmodulin-dependent protein kinase II alpha (CaMKIIα), a serine/threonine kinase highly enriched in the brain, particularly in postsynaptic densities. CaMKIIα plays a central role in synaptic plasticity, learning, and memory by translating calcium signals into long-term changes in neuronal activity. It is activated by calcium/calmodulin binding and autophosphorylation, enabling sustained kinase activity even after calcium levels drop. This antibody specifically detects the α isoform of CaMKII, which is neuron-specific and differs from the β isoform in subcellular localization and function. Researchers use it in techniques like Western blotting, immunohistochemistry, and immunofluorescence to map CaMKIIα expression in brain regions like the hippocampus and cortex, or to investigate dysregulation in neurological disorders such as Alzheimer's disease, epilepsy, and schizophrenia. Many commercial Camk2a antibodies are raised against epitopes in the C-terminal regulatory domain, ensuring specificity. Validation often includes testing on Camk2a knockout mouse tissues. Its applications extend to behavioral studies, where CaMKIIα knockout models show impaired spatial memory, underscoring its functional significance. Proper controls are essential due to potential cross-reactivity with other CaMKII isoforms in non-neuronal tissues.

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