| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Tyrosine-protein kinase Tec, Tec |
| Entrez GeneID | 21682 |
| WB Predicted band size | 73.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Tec antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-205 amino acids from the Central region of mouse Tec. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与Mouse Tec激酶家族抗体相关的虚构参考文献示例(仅供格式参考)：
1. **"Tec kinase regulates B-cell receptor signaling in murine B-lymphocytes"**
*Authors: Smith J, et al. (2018)*
摘要：研究使用Mouse Tec特异性抗体通过Western blot和免疫荧光分析Tec激酶在B细胞活化中的表达定位，发现其通过调控BCR信号通路影响B细胞增殖。
2. **"Expression profiling of Tec family kinases in mouse lung adenocarcinoma models"**
*Authors: Lee H, et al. (2020)*
摘要：通过抗Mouse Tec抗体进行免疫组化分析，证实Tec在肺癌模型肿瘤微环境中的巨噬细胞异常高表达，提示其可能参与肿瘤免疫逃逸机制。
3. **"Genetic ablation of Tec kinase alters T-cell mediated immunity in mice"**
*Authors: Gonzalez R, et al. (2019)*
摘要：采用Tec基因敲除小鼠模型，结合抗Tec抗体进行流式细胞术验证，揭示Tec缺陷导致CD4+ T细胞IL-2分泌减少及Th1分化受损。
4. **"Development of high-affinity monoclonal antibodies against murine Tec kinase for diagnostic applications"**
*Authors: Wang Y, et al. (2021)*
摘要：报道新型鼠源Tec单克隆抗体的开发，通过ELISA和免疫沉淀验证其特异性，成功应用于小鼠脾脏淋巴细胞亚群的Tec蛋白定量检测。
注：以上文献为模拟示例，实际引用请通过PubMed/Web of Science检索真实文献(关键词：Tec kinase, mouse, antibody)。
Mouse Tec antibodies are immunological tools designed to target proteins belonging to the Tec kinase family, a subgroup of non-receptor tyrosine kinases involved in intracellular signaling. The Tec family, including members like BTK (Bruton's tyrosine kinase), ITK (IL-2-inducible T-cell kinase), and TXK, plays critical roles in immune cell development, activation, and survival. These kinases mediate signals from cell surface receptors (e.g., B-cell receptors, T-cell receptors, cytokine receptors) to regulate processes such as proliferation, differentiation, and apoptosis. Dysregulation of Tec kinases is linked to immune disorders, cancers, and inflammatory diseases.
Mouse Tec antibodies are typically developed using mouse-derived antigens or recombinant mouse Tec proteins as immunogens. They are widely used in research to detect endogenous Tec kinases in mouse models, enabling studies on immune cell signaling pathways, hematopoietic malignancies, and autoimmune conditions. Applications include Western blotting, immunohistochemistry, flow cytometry, and functional assays (e.g., kinase activity inhibition). Many commercially available Mouse Tec antibodies are monoclonal, offering high specificity, and are validated for cross-reactivity with homologous human or rat proteins. Their development has advanced mechanistic insights into Tec kinase biology and therapeutic targeting, particularly in contexts like B-cell lymphomas (e.g., BTK inhibitors in clinical use). Proper validation remains essential due to potential isoform cross-reactivity or epitope similarity within the kinase family.
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