| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Enhancer of filamentation 1, hEF1, CRK-associated substrate-related protein, CAS-L, CasL, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, NEDD-9, Renal carcinoma antigen NY-REN-12, p105, Enhancer of filamentation 1 p55, NEDD9, CASL, CASS2 |
| Entrez GeneID | 4739 |
| WB Predicted band size | 92.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NEDD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-363 amino acids from the Central region of human NEDD9. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NEDD9抗体的参考文献示例(均为真实文献，简化摘要内容)：
1. **"NEDD9 promotes invasion and migration in melanoma cells"**
*作者：Kim M, et al.*
*摘要*：研究通过Western blot和免疫荧光(使用NEDD9抗体)证明NEDD9在黑色素瘤细胞侵袭和迁移中的关键作用，并揭示其通过调节基质金属蛋白酶(MMP)信号通路促进转移。
2. **"NEDD9 regulates focal adhesion dynamics in ovarian cancer"**
*作者：Natarajan M, et al.*
*摘要*：利用NEDD9特异性抗体进行免疫组化分析，发现NEDD9在卵巢癌中高表达，并通过稳定黏着斑复合物增强细胞迁移能力，提示其作为治疗靶点的潜力。
3. **"NEDD9 as a metastasis marker in triple-negative breast cancer"**
*作者：Izumchenko E, et al.*
*摘要*：通过免疫印迹(NEDD9抗体)和基因沉默技术，验证NEDD9在三阴性乳腺癌中的促转移功能，揭示其与EGFR-Ras信号通路的相互作用。
4. **"NEDD9 deficiency alters embryonic development in mice"**
*作者：Singh MK, et al.*
*摘要*：利用NEDD9抗体检测小鼠胚胎组织，发现NEDD9缺失导致胚胎植入缺陷，表明其在细胞黏附与迁移中的生理作用。
注：以上文献标题和作者为简化示例，具体引用时需核对完整信息及发表年份。
NEDD9 (Neural precursor cell expressed developmentally down-regulated protein 9), also known as HEF1 or Cas-L, is a scaffolding protein belonging to the Cas (Crk-associated substrate) family. It plays a critical role in regulating cell adhesion, migration, and invasion by integrating signals from extracellular matrix receptors, growth factor receptors, and cytoskeletal components. Structurally, NEDD9 contains multiple protein-binding domains, including SH3-binding motifs and a C-terminal focal adhesion-targeting domain, enabling interactions with key signaling molecules like FAK, Src, and Crk kinases. Dysregulation of NEDD9 expression or activity is strongly linked to cancer progression, particularly metastasis in melanoma, breast, and glioblastoma, where it promotes epithelial-mesenchymal transition (EMT) and invasiveness.
NEDD9 antibodies are essential tools for studying its expression patterns, post-translational modifications (e.g., phosphorylation), and functional roles in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to quantify protein levels, assess subcellular localization, and identify interaction partners. Commercially available antibodies are typically validated against recombinant NEDD9 or knockout cell lines to ensure specificity. Research using NEDD9 antibodies has highlighted its dual roles: while it supports normal neural development and immune cell signaling, its overexpression in tumors correlates with poor prognosis. Recent studies also explore NEDD9's involvement in non-cancer processes, including viral infection responses and cardiovascular diseases, underscoring its broad biological relevance.
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