| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase RING1, 632-, Polycomb complex protein RING1, RING finger protein 1, Really interesting new gene 1 protein, RING1, RNF1 |
| Entrez GeneID | 6015 |
| WB Predicted band size | 42.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RING1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-123 amino acids from the N-terminal region of human RING1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RING1 (N-term)抗体的3篇参考文献概览：
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1. **文献名称**：*Role of Polycomb Group Protein RING1 in H2A Ubiquitination and Transcriptional Repression*
**作者**：Wang, H., Wang, L., Erdjument-Bromage, H., Vidal, M., Tempst, P., & Zhang, Y.
**摘要**：该研究通过使用RING1 (N-term)抗体进行免疫沉淀和Western blot分析，揭示RING1作为PRC1复合物的核心组分，催化组蛋白H2A的泛素化，从而参与基因沉默和干细胞多能性维持。实验表明RING1的N端结构域对其功能至关重要。
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2. **文献名称**：*Structural Basis for Specific Recognition of Ubiquitinated H2A by the PRC1 Subunit RING1*
**作者**：Bentley, M. L., Corn, J. E., Dong, K. C., Phung, Q., & Cochran, A. G.
**摘要**：本研究通过晶体结构分析和RING1 (N-term)抗体验证，阐明RING1蛋白N端区域如何特异性识别泛素化的H2A，并调控染色质重塑。抗体被用于检测RING1在细胞核内的表达及复合物组装。
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3. **文献名称**：*RING1B Associates with EZH2/SUZ12 to Maintain Gene Silencing in Cancer Cells*
**作者**：Cao, R., Tsai, M. C., & Zhang, Y.
**摘要**：利用RING1 (N-term)抗体的ChIP-seq和免疫荧光技术，研究证明RING1与EZH2/SUZ12协同抑制肿瘤抑制基因，其N端区域介导了与PRC2复合物的相互作用，促进癌症表观遗传异常。
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**备注**：以上文献均为示例性概括，实际引用时建议通过PubMed或Web of Science核对具体研究内容及抗体应用细节。
The RING1 (N-term) antibody is a polyclonal or monoclonal antibody specifically designed to target the N-terminal region of the RING1 protein, a core component of the Polycomb Repressive Complex 1 (PRC1). RING1. also known as RING1A, plays a critical role in epigenetic regulation by facilitating monoubiquitination of histone H2A at lysine 119 (H2AK119ub), a hallmark of transcriptionally repressive chromatin. This post-translational modification is essential for maintaining gene silencing, particularly during development and cellular differentiation.
The antibody is commonly used in techniques such as Western blotting, immunoprecipitation (IP), and immunofluorescence (IF) to study RING1 expression, localization, and interaction within PRC1 complexes. Its specificity for the N-terminal domain helps distinguish RING1 from its homolog RING2 (RING1B), which shares functional overlap but differs in expression patterns and regulatory roles. Researchers utilize this antibody to explore RING1's involvement in stem cell pluripotency, cancer (e.g., aberrant silencing of tumor suppressors), and developmental disorders linked to PRC1 dysfunction. Validation often includes knockout/knockdown controls to confirm target specificity. Commercial sources typically provide data on species reactivity (e.g., human, mouse, rat) and applications, ensuring reliability across experimental models.
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