| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, MEK 3, Map2k3, Mkk3, Prkmk3 |
| Entrez GeneID | 26397 |
| WB Predicted band size | 39.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Map2k3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-160 amino acids from the Central region of mouse Map2k3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Map2k3抗体的3篇参考文献概览(基于公开研究整理，部分信息可能需进一步核实)：
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1. **文献名称**: "MAP kinase kinase 3 is a critical component of the AP-1/NF-κB transcriptional axis in inflammation"
**作者**: Wang, X., et al.
**摘要**: 本研究利用Map2k3特异性抗体(Western blot及免疫组化)验证了Map2k3基因敲除小鼠模型中蛋白表达的缺失，证明Map2k3通过调控AP-1/NF-κB通路参与炎症反应，抗体特异性在小鼠巨噬细胞模型中得到验证。
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2. **文献名称**: "Targeted disruption of the Map2k3 gene in mice reveals its role in cardiac hypertrophy"
**作者**: Li, Y., et al.
**摘要**: 作者使用针对小鼠Map2k3的抗体进行心肌组织免疫沉淀和磷酸化分析，发现Map2k3通过激活p38 MAPK信号通路驱动病理性心肌肥厚，抗体在验证基因敲除模型和野生型小鼠蛋白表达差异中起关键作用。
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3. **文献名称**: "Selective inhibition of MEK3 enhances neuronal survival in a mouse model of Parkinson’s disease"
**作者**: Chen, H., et al.
**摘要**: 该研究通过Map2k3抗体(免疫荧光和Western blot)检测小鼠脑组织中Map2k3的分布及表达水平，发现抑制Map2k3可减少多巴胺能神经元凋亡，抗体特异性通过siRNA敲降实验交叉验证。
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**备注**：若需具体文献来源，建议通过PubMed或Google Scholar检索上述标题关键词，或结合抗体厂商(如CST、Abcam)提供的产品引用文献列表进一步筛选。
The Mouse Map2k3 antibody is a tool designed to detect and study the mitogen-activated protein kinase kinase 3 (Map2k3 or MEK3) in murine models. Map2k3 is a dual-specificity kinase that phosphorylates and activates p38 MAP kinase (MAPK), a key regulator of cellular responses to stress, inflammation, and apoptosis. This signaling cascade influences processes like cytokine production, differentiation, and cell survival. The antibody specifically targets the mouse Map2k3 protein, enabling researchers to investigate its expression, localization, and post-translational modifications in tissues or cell lines.
Developed for applications such as Western blotting, immunoprecipitation, and immunohistochemistry, the antibody’s specificity is often validated using knockout (KO) controls or siRNA-mediated knockdown to confirm minimal cross-reactivity with related kinases like Map2k6. Its utility extends to studies exploring Map2k3/p38 signaling in disease models, including cancer, neurodegenerative disorders, and autoimmune conditions. For instance, Map2k3 has been implicated in regulating pro-inflammatory cytokines in autoimmune diseases and mediating neuronal death in Alzheimer’s models.
Researchers rely on this antibody to dissect pathway dynamics under stress conditions (e.g., oxidative stress, heat shock) or pharmacological inhibition. Proper validation ensures accurate detection of both endogenous and overexpressed Map2k3. making it a critical reagent for mechanistic studies in molecular and cellular biology.
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