| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerases I, II, and III subunit RPABC1, RNA polymerases I, II, and III subunit ABC1, DNA-directed RNA polymerase II 23 kDa polypeptide, DNA-directed RNA polymerase II subunit E, RPB5 homolog, XAP4, POLR2E |
| Entrez GeneID | 5434 |
| WB Predicted band size | 24.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This POLR2E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human POLR2E. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于POLR2E (N-term)抗体的3篇参考文献示例：
1. **"Monoclonal antibody targeting POLR2E N-terminal domain reveals its role in transcription elongation"**
- 作者：Chen, X. et al.
- 摘要：研究报道了一种针对POLR2E N端的单克隆抗体的开发，并通过免疫沉淀和ChIP实验证明其在转录延伸复合体中的作用，揭示了POLR2E与RNA聚合酶II其他亚基的相互作用。
2. **"Characterization of RNA polymerase II subunit RPB5 (POLR2E) using domain-specific antibodies"**
- 作者：Kimura, H. et al.
- 摘要：通过针对POLR2E不同结构域的抗体(包括N端)进行Western blot和免疫荧光分析，验证了POLR2E在细胞核内的定位及其在应激条件下转录调控中的功能。
3. **"POLR2E antibody screening identifies dysregulation in cancer cell proliferation"**
- 作者：Wang, L. et al.
- 摘要：利用POLR2E N端抗体筛选多种癌细胞系，发现POLR2E表达水平与肿瘤增殖相关，其缺失导致RNA聚合酶II活性降低和细胞周期停滞。
4. **"Structural insights into the human RNA polymerase II complex by cryo-EM using subunit-specific antibodies"**
- 作者：Fernández-Tornero, C. et al.
- 摘要：研究使用包括POLR2E N端抗体在内的亚基特异性抗体，通过冷冻电镜解析RNA聚合酶II的全结构，阐明了POLR2E在复合体组装中的关键作用。
(注：上述文献为示例，实际引用时需根据真实论文调整。)
The POLR2E (N-term) antibody targets the N-terminal region of RNA Polymerase II Subunit E (POLR2E), a crucial component of the RNA polymerase II (Pol II) complex responsible for mRNA synthesis in eukaryotes. POLR2E, also known as RPB5. is a shared subunit among RNA polymerases I, II, and III, playing a role in transcription initiation and elongation. It interacts with transcriptional regulators and may contribute to the structural integrity of the polymerase core.
This antibody is commonly used to study Pol II assembly, transcriptional regulation, and its dysregulation in diseases like cancer or neurodegenerative disorders. Researchers employ it in techniques such as Western blotting, immunoprecipitation (IP), or chromatin immunoprecipitation (ChIP) to investigate POLR2E expression, localization, and protein-protein interactions. The N-terminal specificity helps distinguish POLR2E from homologous subunits in other RNA polymerases or isoforms. Its applications extend to exploring transcription-coupled DNA repair mechanisms and viral-host interactions, as some pathogens hijack Pol II for replication. Validation typically includes testing on cell lysates with known POLR2E expression and verifying expected molecular weights (~25 kDa). Proper controls ensure specificity, avoiding cross-reactivity with related subunits.
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