| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPOCK1 |
| Uniprot No | Q08629 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-439aa |
| 氨基酸序列 | MPAIAVLAAAAAAWCFLQVESRHLDALAGGAGPNHGNFLDNDQWLSTVSQYDRDKYWNRFRDDDYFRNWNPNKPFDQALDPSKDPCLKVKCSPHKVCVTQDYQTALCVSRKHLLPRQKKGNVAQKHWVGPSNLVKCKPCPVAQSAMVCGSDGHSYTSKCKLEFHACSTGKSLATLCDGPCPCLPEPEPPKHKAERSACTDKELRNLASRLKDWFGALHEDANRVIKPTSSNTAQGRFDTSILPICKDSLGWMFNKLDMNYDLLLDPSEINAIYLDKYEPCIKPLFNSCDSFKDGKLSNNEWCYCFQKPGGLPCQNEMNRIQKLSKGKSLLGAFIPRCNEEGYYKATQCHGSTGQCWCVDKYGNELAGSRKQGAVSCEEEQETSGDFGSGGSVVLLDDLEYERELGPKDKEGKLRVHTRAVTEDDEDEDDDKEDEVGYIW |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPOCK1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *SPOCK1 promotes the invasion and metastasis of gastric cancer through Slug-induced epithelial-mesenchymal transition*
**作者**: Li X, Liu Y, Zhang X, et al.
**摘要**: 本研究通过构建SPOCK1重组蛋白，发现其在胃癌细胞中过表达可通过激活Slug信号通路诱导上皮-间质转化(EMT)，从而促进肿瘤细胞的侵袭和转移。实验表明，SPOCK1重组蛋白处理显著增强胃癌细胞的迁移能力。
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2. **文献名称**: *Recombinant SPOCK1 as a novel regulator of extracellular matrix remodeling in hepatocellular carcinoma*
**作者**: Wang L, Chen J, Zhang H, et al.
**摘要**: 作者利用重组SPOCK1蛋白探究其在肝细胞癌中的作用，发现其通过调控MMP2/9的活性促进细胞外基质降解，加速肿瘤血管生成和侵袭。重组蛋白的体外实验证实其对肝癌细胞迁移具有剂量依赖性促进作用。
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3. **文献名称**: *SPOCK1 interacts with TGF-β1 signaling to modulate astrocyte reactivity in glioblastoma*
**作者**: Kim S, Park J, Lee Y, et al.
**摘要**: 该研究使用重组SPOCK1蛋白分析其在胶质母细胞瘤微环境中的作用，发现SPOCK1与TGF-β1协同激活星形胶质细胞，促进炎症因子释放和肿瘤进展。重组蛋白的应用进一步验证了其在体外模型中的促炎机制。
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(注：以上文献信息为示例性内容，实际引用时需核对真实数据库如PubMed或Web of Science的原文信息。)
**Background of SPOCK1 Recombinant Protein**
SPOCK1 (Secreted Protein Acidic and Cysteine-Rich-like 1), also known as Testican-1. is a calcium-binding extracellular matrix glycoprotein belonging to the SPARC/osteonectin family. It is encoded by the *SPOCK1* gene and is characterized by its modular structure, which includes follistatin-like, calcium-binding EGF-like, and extracellular calcium-binding domains. SPOCK1 interacts with components of the extracellular matrix (ECM) and cell surface receptors, modulating cell-matrix adhesion, migration, proliferation, and tissue remodeling.
Recombinant SPOCK1 protein is engineered using expression systems like *E. coli* or mammalian cells to produce a purified, biologically active form for research. Its production enables precise study of SPOCK1's roles in physiological and pathological processes. SPOCK1 is implicated in cancer progression, neurodevelopment, and inflammatory diseases. In oncology, it promotes epithelial-mesenchymal transition (EMT), metastasis, and therapy resistance in cancers such as glioblastoma, breast, and colorectal cancer by regulating ECM dynamics and signaling pathways (e.g., PI3K/AKT, Wnt/β-catenin).
In neurological contexts, SPOCK1 influences synaptic plasticity and neuronal survival, with altered expression linked to Alzheimer’s disease and autism spectrum disorders. Its recombinant form is also used to investigate interactions with proteoglycans, growth factors, and integrins, offering insights into disease mechanisms and therapeutic targeting. Studies using SPOCK1 recombinant protein highlight its dual role as both a tumor promoter and neuroprotective agent, underscoring its context-dependent functions.
Overall, SPOCK1 recombinant protein serves as a critical tool for deciphering its molecular mechanisms and potential applications in diagnostics or targeted therapies.
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