| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | EphA7 |
| Uniprot No | Q8R381 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 580-994aa |
| 氨基酸序列 | FMVFGFIIGRRHCGYSKADQEGDEELYFHCTKTYIDPETYEDPNRAVHQFAKELDASCIKIERVIGAGEFGEVCSGRLKLPGKRDVAVAIKTLKVGYTEKQRRDFLCEASIMGQFDHPNVVHLEGVVTRGKPVMIVIEFMENGALDAFLRKHDGQFTVIQLVGMLRGIAAGMRYLADMGYVHRDLAARNILVNSNLVCKVSDFGLSRVIEDDPEAVYTTTGGKIPVRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPAPMDCPAGLHQLMLDCWQKDRAERPKFEQIVGILDKMIRNPSSLKTPLGTCSRPISPLLDQSTPDFTAFCSVGEWLQAIKMERYKDNFTAAGYNSLESVARMTIDDVMSLGITLVGHQKKIMSSIQTMRAQMLHLHGTGIQV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于我知识库中信息的模拟参考文献示例(实际文献请通过学术数据库查询)：
1. **文献名称**：Expression and functional analysis of recombinant EphA7 tyrosine kinase domain
**作者**：Smith AB, et al.
**摘要**：报道了人源EphA7酪氨酸激酶结构域的重组表达及纯化，通过体外磷酸化实验验证其激酶活性，并分析了ATP结合位点突变对功能的影响。
2. **文献名称**：EphA7 ectodomain structure reveals mechanism of ligand binding and clustering
**作者**：Jones CD, et al.
**摘要**：通过X射线晶体学解析EphA7重组胞外段结构，揭示了其与ephrin-A5配体的特异性结合模式及受体二聚化机制。
3. **文献名称**：EphA7 regulates neural progenitor cell migration via Rho GTPase signaling
**作者**：Wang L, et al.
**摘要**：利用重组EphA7蛋白刺激神经祖细胞，证明其通过激活RhoA/ROCK通路调控细胞迁移，并发现胞内SAM结构域的关键作用。
4. **文献名称**：Targeting EphA7 with a novel recombinant antibody inhibits tumor growth in xenograft models
**作者**：Zhang Y, et al.
**摘要**：开发了针对EphA7胞外段的单克隆抗体，通过重组蛋白验证结合特异性，并在结肠癌小鼠模型中显示抗肿瘤活性。
**提示**：建议通过PubMed或Google Scholar搜索关键词 "Recombinant EphA7 protein" "EphA7 structure/function" 获取最新文献，部分开放获取文献可能包含详细方法学描述。
EphA7 is a member of the Eph receptor family, the largest subgroup of receptor tyrosine kinases (RTKs), which play critical roles in cell-cell communication and tissue patterning during development. Named after the erythropoietin-producing hepatocellular carcinoma cell line where Eph receptors were first identified, EphA7 specifically binds to ephrin-A ligands, initiating bidirectional signaling pathways that regulate cell adhesion, migration, and boundary formation. It is prominently involved in neural development, including axon guidance, synaptic plasticity, and the establishment of topographic maps in the brain. Dysregulation of EphA7 has been linked to cancer progression, neurodegenerative diseases, and vascular disorders, highlighting its dual roles in physiological and pathological contexts.
Recombinant EphA7 protein is engineered to study its structure-function relationships and signaling mechanisms in vitro or in vivo. Typically produced in mammalian expression systems (e.g., HEK293 or CHO cells), the recombinant protein retains post-translational modifications essential for ligand binding and activation. It often includes the extracellular domain fused to an Fc tag or other solubility-enhancing tags to stabilize the protein and facilitate detection in assays. Purification methods like affinity chromatography ensure high purity and bioactivity. Researchers use recombinant EphA7 to investigate its interactions with ephrins, screen therapeutic agents targeting Eph-ephrin pathways, or model developmental and disease processes. Its applications extend to structural studies (e.g., crystallography) and biomarker discovery, offering insights into therapeutic strategies for cancers or neurological conditions associated with EphA7 signaling anomalies.
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