| 纯度 | >85%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | EphA6 |
| Uniprot No | Q62413 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-546aa |
| 氨基酸序列 | MGGCEVREFL LQFGFFLPLL TAWTGDCSHV SNQVVLLDTT TVMGELGWKT YPLNGWDAIT EMDEHNRPIH TYQVCNVMEP NQNNWLRTNW ISRDAAQKIY VEMKFTLRDC NSIPWVLGTCKETFNLYYI ESDESHGTKF KPSQYIKIDT IAADESFTQM DLGDRILKLN TEIREVGPIE RKGFYLAFQD IGACIALVSV RVFYKKCPFT VRNLAMFPDT IPRVDSSSLV EVRGSCVKSA EERDTPKLYC GADGDWLVPL GRCICSTGYE EIEGSCHACR PGFYKAFAGN TKCSKCPPHS STYVEATSVC HCEKGYFRAE KDPPSMACTR PPSAPRNVAF NINETALILE WSPPSDTGGR KDLTYSVICK KCGLDTTQCE DCGGGLRFIP RHTGLINNSV VVLDFVSHVN YTFEIEAMNG VSELSISPKP FTAITVTTDH DAPSLIGMMR KDWASQNSLA LSWQAPAFSN GAILDYEIKY YEKEHEQLTY SSTRSKAPSV IVTGLKPATT YIFHIRVRTA TGYSGYSQKF EFETGDETSD MAAEQGQ |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于EphA6重组蛋白的模拟参考文献(示例为假设性描述，具体文献需通过学术数据库核实)：
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1. **文献名称**：*"Expression and Functional Characterization of Recombinant EphA6 in Cancer Cell Migration"*
**作者**：Chen L, et al.
**摘要**：研究通过哺乳动物表达系统成功制备了具有生物活性的EphA6重组蛋白，并证明其通过调控下游Rho GTPase信号通路抑制乳腺癌细胞的迁移和侵袭能力，为靶向EphA6的肿瘤治疗提供依据。
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2. **文献名称**：*"Structural Insights into EphA6 Epitope Recognition by a Novel Monoclonal Antibody"*
**作者**：Wang Y, et al.
**摘要**：利用重组EphA6胞外域蛋白筛选出特异性单克隆抗体，通过X射线晶体学解析抗体-抗原复合物结构，揭示了EphA6配体结合域的关键表位，为开发靶向EphA6的诊疗工具奠定基础。
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3. **文献名称**：*"EphA6 Recombinant Protein Modulates Synaptic Plasticity in Hippocampal Neurons"*
**作者**：Smith J, et al.
**摘要**：研究在大肠杆菌中表达并纯化EphA6胞外段重组蛋白，体外实验表明该蛋白可通过反向信号激活神经元突触后信号，调节海马神经元树突棘形态，提示EphA6在神经发育中的潜在作用。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“EphA6 recombinant protein”获取。
EphA6 is a member of the Eph receptor tyrosine kinase family, which plays critical roles in cell-cell communication during development and tissue homeostasis. The Eph receptors are divided into two subclasses (EphA and EphB) based on sequence homology and binding preferences for their ligands, ephrins. EphA6 primarily interacts with GPI-anchored ephrin-A ligands to mediate bidirectional signaling, influencing cell adhesion, migration, and spatial organization. It is highly expressed in the nervous system, particularly in brain regions like the hippocampus and cerebral cortex, where it contributes to axon guidance, synaptic plasticity, and neural circuit formation. Dysregulation of EphA6 has been implicated in neurological disorders and cancers, highlighting its dual role in physiological and pathological processes.
Recombinant EphA6 protein is engineered through molecular cloning and expression systems (e.g., mammalian, insect, or bacterial cells) to produce a purified, functional form of the receptor. This typically includes the extracellular domain, which retains ligand-binding capacity, fused with tags (e.g., Fc, His, or GST) for detection and purification. Researchers utilize recombinant EphA6 to study receptor-ligand interactions, signaling pathways, and its role in diseases. For example, in cancer research, EphA6 overexpression is linked to tumor progression, making it a potential therapeutic target. The protein is also used to generate antibodies, screen drug candidates, or analyze structural features via techniques like X-ray crystallography. Its applications extend to developmental biology models, where EphA6-ephrin dynamics regulate tissue patterning and morphogenesis. Overall, recombinant EphA6 serves as a vital tool for dissecting its biological functions and exploring clinical interventions.
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