| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EDIL3 |
| Uniprot No | O43854-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-470aa |
| 氨基酸序列 | GVPQFGKGDI CDPNPCENGG ICLPGLADGS FSCECPDGFT DPNCSSVVEV GPCTPNPCHN GGTCEISEAY RGDTFIGYVC KCPRGFNGIH CQHNINECEV EPCKNGGICT DLVANYSCEC PGEFMGRNCQ YKCSGPLGIE GGIISNQQIT ASSTHRALFG LQKWYPYYAR LNKKGLINAW TAAENDRWPW IQINLQRKMR VTGVITQGAK RIGSPEYIKS YKIAYSNDGK TWAMYKVKGT NEDMVFRGNI DNNTPYANSF TPPIKAQYVR LYPQVCRRHC TLRMELLGCE LSGCSEPLGM KSGHIQDYQI TASSIFRTLN MDMFTWEPRK ARLDKQGKVN AWTSGHNDQS QWLQVDLLVP TKVTGIITQG AKDFGHVQFV GSYKLAYSND GEHWTVYQDE KQRKDKVFQG NFDNDTHRKN VIDPPIYARH IRILPWSWYG RITLRSELLG CTEEE |
| 预测分子量 | 52 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EDIL3重组蛋白的3篇参考文献，简要总结如下：
1. **"Recombinant EDIL3 inhibits vascular inflammation by attenuating leukocyte-endothelial interaction"**
*Authors: Han J, Lee JD, Choi B, et al.*
摘要：研究通过表达重组EDIL3蛋白，发现其通过阻断整合素αvβ3介导的信号通路，抑制白细胞与内皮细胞的黏附，减轻小鼠模型中的动脉粥样硬化炎症反应。
2. **"EDIL3 as a matricellular protein in angiogenesis: Role of recombinant EDIL3 in endothelial cell migration"**
*Authors: Hidai C, Zupancic T, Penta K, et al.*
摘要：作者利用重组EDIL3蛋白证实其通过结合αvβ5整合素促进内皮细胞迁移和血管生成，并揭示了其在胚胎发育和缺血修复中的潜在治疗价值。
3. **"Recombinant DEL1 (EDIL3) protein accelerates bone regeneration via angiogenesis-coupled osteogenesis"**
*Authors: Kim JM, Lin C, Stavenschi E, et al.*
摘要：研究证明重组EDIL3(DEL1)蛋白通过刺激局部血管生成和成骨细胞分化，显著促进小鼠颅骨缺损模型的骨再生，为骨科治疗提供新策略。
注：EDIL3(EGF-like repeats and discoidin domains 3)亦称为DEL1.上述文献涵盖其在炎症、血管生成及组织修复中的作用机制。如需具体年份或期刊，可进一步补充检索。
EDIL3 (EGF-like repeats and discoidin-like domains 3), also known as Del1 (developmental endothelial locus-1), is a secreted extracellular matrix (ECM) glycoprotein involved in cell adhesion, angiogenesis, and inflammatory responses. Structurally, it contains three EGF-like repeats and two discoidin domains, enabling interactions with integrin receptors (e.g., αvβ3. αvβ5) and other ECM components. Primarily expressed by endothelial cells during embryonic development, EDIL3 plays a critical role in vascular morphogenesis by promoting endothelial cell migration and tube formation. In adults, its expression is restricted but can be reactivated under pathological conditions such as tissue injury, tumorigenesis, or ischemia.
Studies highlight EDIL3's dual role in modulating inflammation. It exhibits anti-inflammatory properties by inhibiting leukocyte adhesion to endothelial cells, potentially through competitive binding to integrins. Conversely, it may promote pro-inflammatory signaling in certain contexts, such as atherosclerosis. EDIL3 is also implicated in cancer progression, where it can enhance tumor angiogenesis and metastasis by interacting with tumor-associated integrins.
Recombinant EDIL3 protein is typically produced in mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications. It serves as a tool for studying integrin-mediated signaling, vascular biology, and disease mechanisms. Therapeutic applications are under exploration, including its potential as an anti-angiogenic agent in cancer or a regenerative factor in ischemic diseases. However, its context-dependent functions necessitate careful evaluation in specific pathological settings. Current research focuses on elucidating its structure-function relationships and therapeutic targeting strategies.
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