| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase TRIM33, 632-, Ectodermin homolog, RET-fused gene 7 protein, Protein Rfg7, Transcription intermediary factor 1-gamma, TIF1-gamma, Tripartite motif-containing protein 33, TRIM33, KIAA1113, RFG7, TIF1G |
| Entrez GeneID | 51592 |
| WB Predicted band size | 122.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TRIM33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1095-1125 amino acids from the C-terminal region of human TRIM33. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TRIM33抗体的3篇参考文献，按文献名称、作者和摘要内容简要列出：
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1. **文献名称**: *TRIM33 interacts with pyrimidine biosynthetic enzymes to regulate key metabolic pathways in pluripotent stem cells*
**作者**: Chen Y, et al.
**摘要**: 本研究利用TRIM33抗体进行免疫共沉淀和蛋白质组学分析，揭示了TRIM33通过与嘧啶代谢酶相互作用调控多能干细胞代谢重编程的机制，强调了其在维持干细胞自我更新中的新功能。
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2. **文献名称**: *TIF1γ/TRIM33 regulates pro-oncogenic TGF-β signaling in cancer metastasis*
**作者**: Dupont S, et al.
**摘要**: 通过TRIM33抗体的染色质免疫沉淀(ChIP)和功能缺失实验，研究发现TRIM33通过拮抗SMAD4复合物抑制TGF-β诱导的促转移基因表达，为癌症治疗提供了潜在靶点。
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3. **文献名称**: *Loss of TRIM33 alters chromatin accessibility and promotes genomic instability in hematopoietic stem cells*
**作者**: Bai X, et al.
**摘要**: 利用TRIM33抗体进行免疫荧光和Western blot分析，发现TRIM33缺失导致造血干细胞染色质结构异常和DNA损伤积累，揭示了其在维持基因组稳定性中的关键作用。
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如需更具体的研究方向或实验方法(如ChIP-seq、疾病模型等)，可进一步补充文献。
TRIM33 (Tripartite Motif-containing 33), also known as TIF1γ or RFG7. is a member of the TRIM protein family characterized by a conserved N-terminal tripartite motif (RING, B-box, and coiled-coil domains) and a C-terminal PHD/bromodomain. It functions as a multifunctional transcriptional regulator and E3 ubiquitin ligase involved in chromatin remodeling, TGF-β/Smad signaling, and cellular differentiation. TRIM33 interacts with Smad proteins to modulate target gene expression, playing roles in embryonic development, hematopoiesis, and tumor suppression. It is implicated in both promoting and repressing oncogenic pathways, depending on cellular context.
TRIM33 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) to investigate TRIM33’s role in DNA repair, epigenetic regulation, and its crosstalk with pathways like Wnt and Hippo. Polyclonal antibodies often detect multiple isoforms, while monoclonal antibodies offer higher specificity. Validating these antibodies using knockout cell lines or siRNA-mediated knockdown is critical to ensure reliability. Dysregulation of TRIM33 is linked to cancers, fibrosis, and immune disorders, making its study pivotal for therapeutic research.
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