| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protocadherin alpha-8, PCDH-alpha-8, PCDHA8 |
| Entrez GeneID | 56140 |
| WB Predicted band size | 103.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PCDHA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-800 amino acids from the C-terminal region of human PCDHA8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PCDHA8抗体的3篇模拟参考文献，格式为文献名称、作者及摘要概括：
1. **"Selective expression of PCDHA8 in developing mouse cerebellum revealed by a novel monoclonal antibody"**
*作者：Tanaka H, et al. (2016)*
摘要：该研究开发了一种针对PCDHA8的小鼠单克隆抗体，验证了其在免疫组化(IHC)和免疫印迹(WB)中的特异性。通过分析胚胎期及出生后小鼠小脑组织，发现PCDHA8在浦肯野细胞中阶段性高表达，提示其可能参与神经元迁移和突触网络建立。
2. **"Proteolytic processing of PCDHA8 modulates synaptic adhesion in hippocampal neurons"**
*作者：Chen L, et al. (2018)*
摘要：研究利用PCDHA8抗体探究其在海马神经元突触中的功能。发现PCDHA8蛋白通过酶切加工释放胞外结构域，影响突触黏附强度，并调节树突棘形态。抗体阻断实验表明PCDHA8对突触可塑性具有调节作用。
3. **"Epigenetic regulation of PCDHA8 expression in autism spectrum disorder models"**
*作者：Smith J, et al. (2020)*
摘要：通过PCDHA8抗体检测自闭症模型小鼠前额叶皮层中的蛋白表达水平，发现DNA甲基化异常导致PCDHA8表达下调，与突触功能缺陷相关。研究提示PCDHA8可能作为神经发育疾病的潜在生物标志物。
注：以上文献为示例性质，实际研究中请通过学术数据库检索真实发表的论文。
The PCDHA8 antibody is a research tool designed to detect Protocadherin alpha 8 (PCDHA8), a member of the clustered protocadherin-alpha (PCDH-α) family. Protocadherins are transmembrane proteins involved in cell-cell adhesion, neuronal development, and synaptic specificity. The PCDH-α cluster, located on human chromosome 5. comprises 12 variable exons (PCDHA1-PCDHA12) and a set of constant exons. PCDHA8 encodes a calcium-dependent cell adhesion protein with six extracellular cadherin repeats, a transmembrane domain, and a cytoplasmic tail. It plays critical roles in neural circuit assembly, axon guidance, and dendrite self-avoidance by mediating homophilic interactions.
Antibodies targeting PCDHA8 are used to study its expression pattern, localization, and function in tissues, particularly the brain. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research involving PCDHA8 antibodies has highlighted its importance in neurodevelopmental disorders and cancer, where dysregulated protocadherin expression is implicated. Specificity validation, such as knockout controls or peptide blocking assays, is essential due to high sequence homology among PCDH-α isoforms. Commercial PCDHA8 antibodies are typically developed in hosts like rabbits or mice, using immunogens derived from conserved regions of the protein. Ongoing studies aim to clarify its role in synaptic plasticity and disease mechanisms.
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