关键信息
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基因名
TNFSF11
- 应用
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别名
Osteoclast differentiation factor;ODF;Osteoprotegerin ligand;OPGL;Receptor activator of nuclear factor kappa-B ligand;RANKL;TNF-related activation-induced cytokine;TRANCE;CD254
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种属
Human
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表达系统
HEK293
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标签
N- His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O14788
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表达区间
63-244aa&linker(NKLLVPRGSPGSGYIPEAPRDGQAYVRKDGEWVLLSTFLG)
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分子量
27.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
TNFSF11, also known as RANKL (Receptor Activator of Nuclear Factor κ B Ligand), is a crucial cytokine in the tumor necrosis factor (TNF) superfamily, primarily involved in the regulation of bone metabolism and immune responses. It plays a pivotal role in osteoclastogenesis, the process by which bone-resorbing osteoclasts are formed from their precursors. The binding of RANKL to its receptor RANK on osteoclast precursors triggers a signaling cascade that leads to the differentiation and activation of these cells, contributing to bone remodeling and homeostasis. Dysregulation of TNFSF11/RANKL signaling has been implicated in various pathological conditions, including osteoporosis, rheumatoid arthritis, and certain malignancies, making it a prime target for therapeutic intervention. Research on recombinant TNFSF11 protein has gained significance in both understanding its biological functions and developing potential treatments for bone-related diseases. By producing and characterizing this protein, scientists can investigate its mechanisms of action, assess its role in disease pathology, and explore its therapeutic potential. Furthermore, recombinant RANKL has been used in preclinical models to study bone biology and in the development of osteoporosis treatments, highlighting its importance in translational research. Overall, the investigation of TNFSF11 in recombinant form serves as a foundational aspect of both basic and applied life sciences, shedding light on its multifaceted roles in health and disease.
