| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Actin-like protein 7A, Actin-like-7-alpha, ACTL7A |
| Entrez GeneID | 10881 |
| WB Predicted band size | 48.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ACTL7A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-67 amino acids from the N-terminal region of human ACTL7A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及ACTL7A(N端)抗体的相关文献摘要信息：
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1. **文献名称**: *"ACTL7A is essential for spermatogenesis in mice"*
**作者**: Li, W., et al.
**摘要**: 该研究利用ACTL7A(N-term)特异性抗体进行Western blot和免疫荧光分析，发现ACTL7A在小鼠睾丸中高表达，敲除后导致精子形态异常和不育，表明其在精子形成中起关键作用。
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2. **文献名称**: *"ACTL7A mutations cause male infertility with teratozoospermia"*
**作者**: Zhang, Y., et al.
**摘要**: 通过ACTL7A N端抗体检测人类精子样本，发现ACTL7A基因突变与畸形精子症相关，其功能缺失影响顶体形成和精子头部形态。
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3. **文献名称**: *"Expression and localization of ACTL7A in human germ cells"*
**作者**: Chen, H., et al.
**摘要**: 研究利用N端特异性抗体进行免疫组化分析，证实ACTL7A在人类精母细胞和圆形精子细胞中特异性表达，提示其参与减数分裂和染色质重塑过程。
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**备注**：以上文献为模拟摘要，实际研究中需通过PubMed或Google Scholar检索具体文献。若需真实文献，建议使用关键词“ACTL7A antibody N-terminal”或结合基因功能(如“spermatogenesis”)进行查询。
The ACTL7A (N-term) antibody is a specific immunological tool designed to target the N-terminal region of Actin-like protein 7A (ACTL7A), a member of the actin-related protein (ARP) family. ACTL7A is a testis-enriched protein implicated in spermatogenesis and male fertility. Unlike conventional actins, ACTL7A lacks intrinsic ATPase activity and is thought to function in specialized cellular processes, such as acrosome formation during sperm development. Its expression is tightly regulated during germ cell differentiation, with studies linking ACTL7A mutations or dysregulation to teratozoospermia and infertility.
This antibody is commonly used in research to study ACTL7A's expression patterns, subcellular localization, and interactions in reproductive tissues or cell models. It enables detection via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Validation typically includes reactivity tests in species of interest (e.g., human, mouse) and knockout controls to confirm specificity. The N-terminal epitope recognition ensures minimal cross-reactivity with other actin isoforms. Researchers utilize this antibody to explore ACTL7A's roles in cytoskeletal dynamics during spermiogenesis, its molecular partnerships (e.g., with other ARPs or acrosomal proteins), and its potential as a biomarker for diagnosing male reproductive disorders. Its development underscores the importance of ACTL7A in understanding gametogenesis and infertility mechanisms.
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