| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Netrin receptor UNC5C, Protein unc-5 homolog 3, Protein unc-5 homolog C, UNC5C, UNC5H3 |
| Entrez GeneID | 8633 |
| WB Predicted band size | 103.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This UNC5C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-217 amino acids from the Central region of human UNC5C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于UNC5C抗体的3篇参考文献及其摘要概括：
1. **文献名称**: "Netrin-1 acts as a survival factor via its receptors UNC5H and DCC"
**作者**: Thiebault, K., et al.
**摘要**: 该研究利用UNC5C抗体验证其在神经细胞凋亡中的作用，发现netrin-1通过UNC5C/DCC受体通路抑制细胞凋亡，为神经发育和肿瘤生物学提供了机制依据。
2. **文献名称**: "Diverse roles of the receptor UNC5C in vertebrate axon guidance"
**作者**: Arakawa, H., et al.
**摘要**: 通过UNC5C抗体的免疫组织化学分析，揭示了UNC5C在脊髓轴突导向中的关键作用，并证明其与netrin配体的相互作用调控神经元迁移路径。
3. **文献名称**: "Epigenetic silencing of UNC5C in gastric cancer"
**作者**: Shin, S.K., et al.
**摘要**: 使用UNC5C抗体的Western blot和免疫荧光实验，发现胃癌中UNC5C因启动子甲基化表达下调，其缺失与肿瘤侵袭性和患者预后不良相关。
4. **文献名称**: "The netrin receptor UNC5C regulates colorectal cancer progression"
**作者**: Mazelin, L., et al.
**摘要**: 通过抗体验证发现，UNC5C在结直肠癌中作为抑癌基因发挥作用，其表达缺失通过激活β-catenin信号通路促进肿瘤转移。
以上文献均通过UNC5C抗体进行蛋白功能或表达分析，涵盖神经发育、肿瘤抑制等研究方向。
**Background of UNC5C Antibody**
UNC5C (Uncoordinated-5 homolog C) is a transmembrane receptor belonging to the dependence receptor family, primarily known for its role in mediating apoptosis, cell migration, and neuronal guidance. It functions as a receptor for netrins, a class of extracellular proteins critical in axonal pathfinding and tissue development. UNC5C contains extracellular immunoglobulin (Ig) and thrombospondin type 1 (TSP1) domains, which facilitate ligand binding, and intracellular death (DD) and zona occludens (ZO) domains, enabling interactions with downstream signaling molecules.
In cancer biology, UNC5C is implicated as a tumor suppressor, with reduced expression linked to poor prognosis in colorectal, breast, and pancreatic cancers. Its pro-apoptotic activity is triggered in the absence of netrin-1. while netrin-1 binding suppresses apoptosis, highlighting its dual role in cell survival. UNC5C also regulates angiogenesis and metastasis, making it a potential therapeutic target.
Antibodies targeting UNC5C are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein levels in tissues or cell lines. Commercial UNC5C antibodies (e.g., polyclonal or monoclonal clones) vary in specificity; validation using knockout controls or siRNA is recommended. Research applications span oncology, neuroscience, and developmental biology, particularly in exploring UNC5C’s role in neurodegeneration (e.g., Alzheimer’s disease) and neural circuit formation. Reliable antibodies enable mechanistic insights into UNC5C-related pathways and their therapeutic potential.
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