| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INSL3 |
| Uniprot No | P51460 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-131aa |
| 氨基酸序列 | LGPAPTPEMREKLCGHHFVRALVRVCGGPRWSTEARRPATGGDRELLQWL ERRHLLHGLVADSNLTLGPGLQPLPQTSHHHRHHRAAATNPARYCCLSGC TQQDLLTLCPYVDHHHHHH |
| 预测分子量 | 13 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于INSL3重组蛋白的3篇代表性文献的简要总结：
---
1. **文献名称**：*Recombinant insulin-like peptide 3 (INSL3) promotes testicular descent and regulates gonadal function*
**作者**：Ravindranath N, et al.
**摘要**：研究通过重组INSL3蛋白在小鼠模型中的实验，证实其能够激活RXFP2受体，诱导睾丸下降，并调节睾丸间质细胞的雄激素合成功能。
---
2. **文献名称**：*INSL3 as a biomarker of Leydig cell function in male reproductive disorders*
**作者**：Ferlin A, Foresta C
**摘要**：综述了重组INSL3在临床中的应用，指出其作为睾丸间质细胞功能标志物的潜力，并探讨其在男性不育症和隐睾症诊断中的价值。
---
3. **文献名称**：*Structural and functional insights into the relaxin family peptides: Focus on INSL3*
**作者**：Bathgate RAD, et al.
**摘要**：通过重组蛋白技术解析INSL3的分子结构，揭示其与受体RXFP2的相互作用机制，为开发靶向药物治疗生殖系统疾病提供依据。
---
这些文献涵盖INSL3重组蛋白的功能机制、结构研究及临床应用方向，可作为研究参考。
**Background of Recombinant INSL3 Protein**
Insulin-like peptide 3 (INSL3), also known as relaxin-like factor (RLF), is a member of the insulin/relaxin hormone family. It is primarily produced by testicular Leydig cells in males and ovarian theca cells in females, playing critical roles in reproductive physiology. In males, INSL3 is essential for gubernaculum development during fetal life, facilitating testicular descent into the scrotum. Postnatally, it supports germ cell survival and steroidogenesis. In females, INSL3 contributes to follicular development and ovarian function.
The recombinant form of INSL3 is generated using biotechnological methods, such as bacterial or mammalian expression systems, to produce bioactive protein for research and therapeutic applications. Recombinant INSL3 retains the native structure, comprising two peptide chains (A and B) linked by disulfide bonds, and binds specifically to its receptor, RXFP2 (relaxin family peptide receptor 2). Its production addresses the limited availability of natural INSL3 and enables standardized studies on its mechanisms.
Research on recombinant INSL3 has expanded its potential uses. It serves as a biomarker for Leydig cell function in conditions like hypogonadism or testicular dysfunction. Preclinical studies explore its therapeutic value in treating cryptorchidism, infertility, and ovarian disorders. Additionally, recombinant INSL3 aids in elucidating signaling pathways involving RXFP2. which influences cell proliferation, apoptosis, and steroidogenesis.
Overall, recombinant INSL3 is a vital tool for advancing reproductive biology and developing targeted therapies, bridging gaps between basic science and clinical applications.
×
