| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMP1 |
| Uniprot No | P11279 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-382aa |
| 氨基酸序列 | AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRS SCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLF PNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAY LSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTN GTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLEL HSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRAL QATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLD ENSMVDHHHHHH |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAMP1重组蛋白的假设性参考文献示例(文献内容基于领域常见研究方向虚构，实际文献需通过数据库检索确认)：
1. **文献名称**：*Expression and Functional Characterization of Recombinant LAMP1 in Lysosomal Storage Disorders*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人源LAMP1蛋白，并验证其促进溶酶体酶活性的功能，为溶酶体贮积症的体外治疗提供潜在分子工具。
2. **文献名称**：*Recombinant LAMP1 Protein Enhances Autophagic Flux in Neurodegenerative Disease Models*
**作者**：Chen X, Wang Y.
**摘要**：通过体外纯化的LAMP1重组蛋白处理阿尔茨海默病细胞模型，发现其显著减少异常蛋白聚集，机制与自噬溶酶体通路的激活相关。
3. **文献名称**：*Structural Analysis of LAMP1 Extracellular Domain Using Recombinant Protein Crystallography*
**作者**：Tanaka K, et al.
**摘要**：解析了重组LAMP1胞外域的高分辨率晶体结构，揭示了其糖基化修饰位点与细胞黏附功能的分子基础，为靶向药物设计提供结构依据。
4. **文献名称**：*LAMP1 Recombinant Protein as a Biomarker for Cancer Immunotherapy Response*
**作者**：Liu H, et al.
**摘要**：在黑色素瘤模型中验证了重组LAMP1蛋白与肿瘤外泌体的结合能力，提出其作为预测PD-1抑制剂疗效的生物标志物潜力。
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**提示**：实际文献需通过PubMed、Web of Science等平台以“LAMP1 recombinant protein”或“LAMP1 purification”等关键词检索，重点关注蛋白表达体系(如哺乳动物/昆虫细胞)、功能验证(如溶酶体定位、免疫共沉淀)及应用场景(疾病模型、诊断试剂开发)。
**Background of LAMP1 Recombinant Protein**
Lysosome-associated membrane protein 1 (LAMP1) is a type I transmembrane glycoprotein predominantly localized in lysosomal membranes, playing a critical role in lysosome biogenesis, autophagy, and cellular trafficking. As a member of the LAMP family, LAMP1 is characterized by a heavily glycosylated luminal domain, which protects lysosomal membranes from degradation, and a short cytoplasmic tail involved in interactions with cytosolic proteins for organelle motility and membrane fusion.
Recombinant LAMP1 proteins are engineered to study its structure-function relationships, post-translational modifications (e.g., glycosylation), and interactions with ligands or pathogens. These proteins are typically produced using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper folding and glycosylation, which are critical for its biological activity. Purification often involves affinity tags (e.g., His-tag) and chromatographic techniques.
Research applications include investigating lysosomal disorders (e.g., Danon disease), neurodegenerative diseases, and cancer, where LAMP1 dysregulation is linked to altered lysosomal function or immune evasion. Recombinant LAMP1 also serves as a tool to study pathogen-host interactions, as some viruses (e.g., SARS-CoV-2) and bacteria exploit LAMP1-positive compartments for entry or survival. Additionally, engineered LAMP1 fusion proteins are used in drug delivery systems targeting lysosomes.
Overall, recombinant LAMP1 provides a versatile platform for advancing our understanding of lysosomal biology, disease mechanisms, and therapeutic development.
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