| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Scarb2 |
| Uniprot No | Q14108 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-432aa |
| 氨基酸序列 | RVFQ KAVDQSIEKK IVLRNGTEAF DSWEKPPLPV YTQFYFFNVT NPEEILRGET PRVEEVGPYT YRELRNKANI QFGDNGTTIS AVSNKAYVFE RDQSVGDPKI DLIRTLNIPV LTVIEWSQVH FLREIIEAML KAYQQKLFVT HTVDELLWGY KDEILSLIHV FRPDISPYFG LFYEKNGTND GDYVFLTGED SYLNFTKIVE WNGKTSLDWW ITDKCNMING TDGDSFHPLI TKDEVLYVFP SDFCRSVYIT FSDYESVQGL PAFRYKVPAE ILANTSDNAG FCIPEGNCLG SGVLNVSICK NGAPIIMSFP HFYQADERFV SAIEGMHPNQ EDHETFVDIN PLTGIILKAA KRFQINIYVK KLDDFVETGD IRTMVFPVMY LNESVHIDKE TASRLKSMIN TT |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCARB2/LIMP-2重组蛋白的模拟参考文献示例(注：内容为假设性概括，建议通过PubMed等数据库获取真实文献)：
1. **标题**: "Recombinant SCARB2 Expression in HEK293 Cells: Purification and Structural Characterization"
**作者**: Zhang L, et al.
**摘要**: 本研究利用HEK293细胞系统表达了重组人SCARB2蛋白，通过亲和层析技术纯化并分析其结构。结果表明，重组蛋白具有与天然SCARB2相似的糖基化修饰和溶酶体靶向功能，为后续功能研究提供工具。
2. **标题**: "SCARB2-Mediated Enterovirus 71 Entry: Role of Recombinant Protein in Viral Binding"
**作者**: Wang Y, et al.
**摘要**: 通过表达重组SCARB2蛋白，验证其作为肠道病毒71型(EV71)的功能性受体。体外实验表明，重组SCARB2与病毒衣壳蛋白VP1特异性结合，阻断该互作可抑制病毒感染。
3. **标题**: "LIMP-2/SCARB2 Deficiency and Lysosomal Dysfunction: Insights from Recombinant Protein Rescue Experiments"
**作者**: Gupta S, et al.
**摘要**: 研究利用重组SCARB2蛋白修复缺乏LIMP-2的细胞模型，发现其恢复溶酶体酶运输功能，证实SCARB2在溶酶体相关疾病(如动作肌阵挛-肾衰竭综合征)中的关键作用。
4. **标题**: "Cryo-EM Structure of Recombinant Human SCARB2 Reveals Cholesterol Binding Domains"
**作者**: Tanaka K, et al.
**摘要**: 通过冷冻电镜解析重组SCARB2的高分辨率结构，发现其跨膜结构域存在胆固醇结合口袋，为理解其在脂质代谢和神经退行性疾病中的机制提供结构基础。
**提示**：实际文献可通过检索关键词“SCARB2 recombinant protein”“LIMP-2 expression”或结合具体研究领域(如病毒感染、溶酶体疾病)在PubMed/Google Scholar中查找。
**Background of SCARB2 Recombinant Protein**
SCARB2 (scavenger receptor class B member 2), also known as LIMP-2 (lysosomal integral membrane protein type 2) or CD36b, is a transmembrane glycoprotein belonging to the CD36 scavenger receptor family. It is primarily localized in lysosomal and endosomal membranes, playing critical roles in cellular processes such as lipid metabolism, membrane trafficking, and lysosomal biogenesis. SCARB2 acts as a receptor for enterovirus 71 (EV71) and coxsackievirus A16 (CVA16), pathogens associated with hand, foot, and mouth disease (HFMD), making it a key target for antiviral research.
Recombinant SCARB2 protein is engineered through heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional protein for biomedical studies. Its recombinant form retains structural and functional properties, enabling researchers to study virus-host interactions, receptor-ligand binding mechanisms, and intracellular trafficking pathways. Additionally, SCARB2 is implicated in lysosomal storage disorders, such as action myoclonus-renal failure syndrome (AMRF), due to mutations affecting lysosomal function.
The production of recombinant SCARB2 facilitates drug discovery, vaccine development, and diagnostic tool design, particularly for combating enteroviral infections. Structural studies using recombinant SCARB2 have revealed insights into its extracellular domains responsible for viral attachment, aiding in the design of entry inhibitors. Furthermore, its role in lipid homeostasis and lysosomal dysfunction links it to broader research on neurodegenerative diseases and metabolic disorders.
Overall, SCARB2 recombinant protein serves as a vital tool for unraveling its biological functions and therapeutic potential in virology, lysosomal biology, and beyond.
×
