| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALDH1A2 |
| Uniprot No | O94788 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-518aa |
| 氨基酸序列 | MTSSKIEMPGEVKADPAALMASLHLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVYNPATGEQVCEVQEADKADIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRAVLATMESLNGGKPFLQAFYVDLQGVIKTFRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVCGQIIPWNFPLLMFAWKIAPALCCGNTVVIKPAEQTPLSALYMGALIKEAGFPPGVINILPGYGPTAGAAIASHIGIDKIAFTGSTEVGKLIQEAAGRSNLKRVTLELGGKSPNIIFADADLDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVRRSVERAKRRVVGSPFDPTTEQGPQIDKKQYNKILELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFGPVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALTVSSAMQAGTVWINCYNALNAQSPFGGFKMSGNGREMGEFGLREYSEVKTVTVKIPQKNS |
| 预测分子量 | 58.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALDH1A2重组蛋白的假设参考文献示例(仅供参考，非真实文献)：
1. **"Expression and characterization of recombinant human ALDH1A2: Role in retinoic acid biosynthesis"**
*作者：Smith J, et al. (2015)*
摘要：研究利用大肠杆菌系统表达重组人ALDH1A2蛋白，纯化后分析其催化视黄醛转化为视黄酸的酶动力学特性，证实其在视黄酸合成中的关键作用。
2. **"Structural insights into ALDH1A2 through recombinant protein crystallography"**
*作者：Lee H, et al. (2020)*
摘要：通过昆虫细胞表达重组ALDH1A2蛋白，解析其三维晶体结构，揭示底物结合口袋的关键氨基酸残基，为设计选择性抑制剂提供结构基础。
3. **"ALDH1A2 recombinant protein promotes stem cell differentiation via retinoic acid signaling"**
*作者：Johnson R, et al. (2018)*
摘要：在HEK293细胞中表达并纯化ALDH1A2重组蛋白，证明其通过生成视黄酸激活下游信号通路，调控胚胎干细胞向神经谱系分化。
4. **"Development of a high-throughput assay for ALDH1A2 activity using purified recombinant protein"**
*作者：Chen L, et al. (2017)*
摘要：建立基于重组ALDH1A2蛋白的高通量酶活检测方法，用于筛选调节其活性的小分子化合物，并应用于癌症代谢研究的药物开发。
**注**：以上文献为模拟示例，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。
ALDH1A2. a member of the aldehyde dehydrogenase 1A subfamily, is a key enzyme in retinoic acid (RA) biosynthesis. It catalyzes the oxidation of retinaldehyde to RA, a critical signaling molecule regulating embryonic development, cell differentiation, and tissue homeostasis. Dysregulation of ALDH1A2 has been linked to developmental disorders, cancer, and neurodegenerative diseases, underscoring its biological and clinical relevance.
Recombinant ALDH1A2 protein is engineered for research and therapeutic applications. Typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, the recombinant form retains enzymatic activity and structural integrity, enabling functional studies. It often includes affinity tags (e.g., His-tag) for simplified purification. Researchers use this protein to investigate RA signaling pathways, screen ALDH1A2-targeting drugs, and model disease mechanisms. For example, its role in cancer stem cell maintenance has prompted studies exploring ALDH1A2 inhibitors as potential anticancer agents. In developmental biology, recombinant ALDH1A2 helps dissect RA-dependent patterning in model organisms.
Structural analyses using recombinant protein have revealed substrate-binding sites and catalytic residues, advancing mechanistic understanding. Additionally, it serves as a standard in diagnostic assays to quantify ALDH1A2 activity in clinical samples. The availability of high-purity recombinant ALDH1A2 accelerates both basic research and drug discovery, offering insights into RA-related pathologies and therapeutic strategies. Its consistent quality and scalability address challenges associated with native protein isolation, ensuring reproducibility in experimental systems.
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