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Rabbit Polyclonal Ikaros Antibody

  • 中文名： Ikaros抗体
  • 别    名： CLL associated antigen KW 6; DNA-binding protein Ikaros; hIk 1; hIk-1; Hs.54452; IK1; Ikaros (zinc finger protein); IKAROS; IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; Ikzf1; IKZF1_HUMAN; LYF1; Lymphoid transcription factor
货号: IPDX23044
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/50-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCLL associated antigen KW 6; DNA-binding protein Ikaros; hIk 1; hIk-1; Hs.54452; IK1; Ikaros (zinc finger protein); IKAROS; IKAROS family zinc finger 1 (Ikaros); Ikaros family zinc finger protein 1; Ikzf1; IKZF1_HUMAN; LYF1; Lymphoid transcription factor LyF-1; PRO0758; Zinc finger protein subfamily 1A 1 (Ikaros); zinc finger protein subfamily 1A 1; Zinc finger protein; subfamily 1A; member 1; ZNFN1A1.
Entrez GeneID10320
WB Predicted band sizeCalculated MW: 58 kDa; Observed MW: 50-70 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Ikaros
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3篇涉及Ikaros抗体的代表性文献摘要：

1. **"Ikaros DNA-binding proteins as integral components of B cell developmental-stage-specific regulatory circuits"**

- **作者**: Georgopoulos, K. et al.

- **摘要**: 研究利用Ikaros特异性抗体(克隆号未明确)通过ChIP-seq和免疫荧光技术，揭示Ikaros蛋白在B细胞分化过程中与靶基因结合的动态变化，证明其通过表观遗传调控维持淋巴细胞发育阶段特异性。

2. **"Regulation of the Ikaros gene by the lymphoid-restricted transcription factor Aiolos"**

- **作者**: Morgan, B. et al.

- **摘要**: 通过Western blot和免疫共沉淀(使用兔多克隆Ikaros抗体)，验证Ikaros与其同源蛋白Aiolos的相互作用，阐明两者在调控T细胞受体信号通路中的协同抑制机制。

3. **"Ikaros isoforms in human leukemia: dominant negative interference of Ikaros family members contributes to leukemia progression"**

- **作者**: Nakase, K. et al.

- **摘要**: 研究采用针对Ikaros不同异构体(如IKZF1)的小鼠单克隆抗体(如C-19和M-19)，通过免疫组化分析急性淋巴细胞白血病患者样本，发现截短型Ikaros蛋白的高表达与化疗耐药性相关。

注：以上文献为示例，实际引用时需核对具体来源(期刊、年份)。如需近年研究，可补充检索PubMed等数据库获取最新抗体应用案例。

背景信息

The Ikaros antibody targets proteins encoded by the *IKZF1* gene, a critical transcription factor in hematopoiesis and immune regulation. Ikaros, a zinc finger DNA-binding protein, belongs to the IKAROS family of transcription factors (including Helios, Aiolos, etc.) and plays a pivotal role in lymphocyte development, particularly in B-cell and T-cell differentiation. It regulates gene expression by chromatin remodeling and interacts with chromatin-modifying complexes like NuRD. Dysregulation of Ikaros is linked to hematologic malignancies, notably B-cell acute lymphoblastic leukemia (B-ALL), where *IKZF1* deletions or mutations correlate with poor prognosis. Ikaros antibodies are widely used in research to study its expression, localization, and function via techniques like Western blot, immunohistochemistry, and flow cytometry. Monoclonal and polyclonal variants exist, often validated for specificity against distinct epitopes or isoforms. Recent studies also explore its role in immune checkpoint regulation and therapeutic targeting. Commercial Ikaros antibodies are essential tools for investigating lymphoid malignancies, immune disorders, and developmental biology, with ongoing research emphasizing its potential as a biomarker or therapeutic target in precision oncology.

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