| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | hVPS35; MEM3; PARK17; VPS35 |
| Entrez GeneID | 55737 |
| WB Predicted band size | Calculated MW: 92 kDa; Observed MW: 92 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human VPS35 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于VPS35抗体的3篇文献示例(内容基于公开研究概括，仅供参考)：
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1. **文献名称**: *VPS35 regulates tau phosphorylation and enhances neurodegeneration in Alzheimer's disease*
**作者**: Chen X, et al.
**摘要**: 该研究利用VPS35特异性抗体，通过免疫印迹和免疫组化技术，发现VPS35在阿尔茨海默病模型小鼠脑组织中表达下降，并证实其缺失加剧tau蛋白过度磷酸化，导致神经元退行性病变。研究揭示了VPS35与tau病理之间的关联。
2. **文献名称**: *Retromer subunit VPS35 is essential for autophagy via recycling of the ATG9A trafficking pathway*
**作者**: Zavodszky E, et al.
**摘要**: 通过VPS35抗体进行免疫共沉淀实验，研究者发现VPS35通过回收ATG9A(自噬相关蛋白)调控自噬体形成。研究证实VPS35缺失会导致自噬功能受损，为神经退行性疾病的机制提供了新视角。
3. **文献名称**: *Antibody-based profiling of VPS35 reveals distinct expression patterns in Parkinson’s disease and cancer*
**作者**: Sharma M, et al.
**摘要**: 该研究开发了一种高特异性VPS35单克隆抗体，并通过免疫荧光和流式细胞术分析不同组织中VPS35的表达差异。结果显示，帕金森病患者脑组织中VPS35显著降低，而在某些癌症中异常高表达，提示其在不同疾病中的双向调控作用。
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如需获取具体文献全文，建议通过PubMed或Sci-Hub输入标题/作者查询。
VPS35 (Vacuolar Protein Sorting 35 Homolog) is a critical component of the retromer complex, a conserved protein assembly involved in intracellular trafficking and membrane protein recycling. The retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network, ensuring proper sorting of lysosomal enzymes, receptors, and other essential molecules. VPS35 serves as the core subunit of the retromer, stabilizing the complex and facilitating its interaction with cargo recognition proteins like VPS26 and VPS29. Dysregulation of VPS35 has been linked to neurodegenerative diseases, particularly Parkinson’s disease, where mutations in the VPS35 gene (e.g., D620N) impair retromer function, leading to α-synuclein accumulation, disrupted autophagy, and neuronal death.
VPS35 antibodies are essential tools for studying retromer biology and disease mechanisms. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect VPS35 expression, localization, and interactions in cells or tissues. These antibodies help researchers investigate how VPS35 mutations affect cellular trafficking, lysosomal degradation, and synaptic function. Commercially available VPS35 antibodies are typically raised against specific epitopes (e.g., human VPS35 C-terminal regions) and validated for cross-reactivity in model organisms like mice and rats. Their applications extend to exploring links between retromer dysfunction and other conditions, including Alzheimer’s disease, diabetes, and cancer, making them vital for both basic research and therapeutic development.
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