| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BAP1; DING; BAP-1; HIPI3; RING2; RING1B |
| Entrez GeneID | 6045 |
| WB Predicted band size | Calculated MW: 38 kDa; Observed MW: 38 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RING2 / RING1B / RNF2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RING2抗体的3篇代表性文献(虚构示例，仅供参考)：
1. **文献名称**：RING2/PCGF4 is essential for Polycomb-mediated gene silencing via H2A ubiquitination
**作者**：G. Buchwald et al.
**摘要**：本研究利用特异性RING2抗体，通过ChIP-seq技术揭示了RING2在PRC1复合体中的核心作用，证明其通过催化组蛋白H2A K119位点的泛素化修饰调控干细胞分化相关基因的沉默。
2. **文献名称**：Antibody-based profiling of RING2 reveals dynamic chromatin interactions in cancer
**作者**：L. Wang et al.
**摘要**：作者开发了一种高特异性RING2单克隆抗体，应用于免疫共沉淀和免疫荧光实验，发现RING2在多种癌细胞中异常富集于抑癌基因位点，提示其作为表观遗传治疗靶点的潜力。
3. **文献名称**：Functional characterization of RING2 antibodies in PRC1 assembly assays
**作者**：N.P. Blackledge et al.
**摘要**：该研究系统比较了多种商业RING2抗体的特异性，发现部分抗体能有效阻断PRC1复合体的体外组装过程，为研究RING2的结构功能提供了关键实验工具。
注：以上文献为模拟示例，实际研究中建议通过PubMed或Google Scholar以“RING2 antibody”、“PCGF4”、“PRC1 H2A ubiquitination”等关键词检索最新文献。
The RING2 protein, also known as RNF2 or Ring1B, is a core component of the Polycomb Repressive Complex 1 (PRC1), a key epigenetic regulator involved in gene silencing. Functioning as an E3 ubiquitin ligase, RING2 catalyzes the monoubiquitination of histone H2A at lysine 119 (H2AK119ub), a modification critical for maintaining transcriptional repression of developmental genes, particularly during cellular differentiation and embryogenesis. RING2 works in conjunction with other PRC1 subunits to establish compact chromatin structures, ensuring stable long-term gene silencing. Its activity is essential for normal development, stem cell maintenance, and X-chromosome inactivation. Dysregulation of RING2 has been implicated in cancers, where aberrant PRC1 function can drive oncogenic programs.
Antibodies targeting RING2 are widely used in research to study PRC1-mediated gene regulation. They enable detection of RING2 expression levels, localization, and interaction partners through techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Specific RING2 antibodies help distinguish it from its homolog RING1A (Ring1A), which shares functional redundancy but exhibits distinct biological roles. These tools are vital for investigating Polycomb group protein dynamics in development, disease, and epigenetic inheritance mechanisms. Validation of antibody specificity remains crucial due to structural similarities within the Polycomb family.
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