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Rabbit Polyclonal CDT2 Antibody

  • 中文名： CDT2抗体
  • 别    名： CDW1; DCAF2; Dtl; L2DTL; RAMP
货号: IPDX23038
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/100 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 1/50-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCDW1; DCAF2; Dtl; L2DTL; RAMP
Entrez GeneID51514
WB Predicted band sizeCalculated MW: 79 kDa; Observed MW: 91 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human CDT2
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于CDT2抗体的3篇参考文献示例(内容基于公开研究综合整理，非真实文献)：

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1. **文献名称**：*CRL4CDT2 ubiquitin ligase regulates cell cycle progression through degradation of p21 and Set8*

**作者**：Higa et al.

**摘要**：该研究利用特异性CDT2抗体，揭示了CRL4-CDT2复合体在DNA损伤后通过泛素化降解p21和Set8蛋白的机制，证明其对S期进程和基因组稳定性的调控作用。抗体用于Western blot和免疫共沉淀实验，验证了CDT2与底物的相互作用。

2. **文献名称**：*CDT2 overexpression promotes tumorigenesis by destabilizing the DNA replication licensing inhibitor Geminin*

**作者**：Kim et al.

**摘要**：通过免疫组化(使用CDT2抗体)分析临床样本，发现CDT2在多种癌症中高表达，并与Geminin蛋白水平负相关。研究证明CDT2通过降解Geminin促进DNA复制异常，推动肿瘤发生。抗体用于组织染色和蛋白定量分析。

3. **文献名称**：*Development of a monoclonal antibody against human CDT2 for functional studies in cell cycle regulation*

**作者**：Zhang et al.

**摘要**：本文报道了一种新型抗人CDT2单克隆抗体的开发，验证了其在高通量筛选和小鼠模型中的特异性。实验显示，该抗体能有效抑制CRL4-CDT2复合体活性，为靶向CDT2的抗癌治疗策略提供了工具。

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**备注**：以上文献为示例，实际研究中建议通过PubMed或Web of Science以“CDT2 antibody”或“CRL4-CDT2”为关键词检索最新论文。如需具体文献，可提供更详细的研究方向或应用场景。

背景信息

CDT2 antibody targets the protein CDT2 (also known as Cullin4-DDB1-binding WD40 protein 2 or DCAF2), a critical substrate receptor component of the CRL4 (Cullin-RING ligase 4) ubiquitin ligase complex. CDT2 plays a pivotal role in cell cycle regulation, DNA replication, and genome stability by promoting the proteasomal degradation of key substrates like p21. SET8. and CDC25A. These substrates are involved in cell cycle checkpoints, chromatin modification, and replication licensing. CDT2 is particularly active during the S phase, ensuring timely degradation of licensing factors to prevent re-replication and maintain genomic integrity. Dysregulation of CDT2 is linked to cancer progression, as overexpression can drive uncontrolled proliferation, chemoresistance, and genomic instability. Antibodies against CDT2 are essential tools for studying its expression, localization, and interaction partners in various cellular contexts. They are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to explore its role in DNA damage responses, replication stress, and tumorigenesis. Research on CDT2 also highlights its potential as a therapeutic target, with inhibitors under investigation to disrupt CRL4-CDT2 activity in cancers. Understanding CDT2's regulatory mechanisms through antibody-based assays remains vital for elucidating its contributions to cell cycle control and disease pathology.

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