| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Aspartate aminotransferase; cytoplasmic; Glutamate oxaloacetate transaminase 1; Transaminase A |
| Entrez GeneID | 2805 |
| WB Predicted band size | Calculated MW: 46 kDa; Observed MW: 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Aspartate Aminotransferase |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇与Aspartate Aminotransferase(AST)抗体相关的代表性文献摘要：
---
1. **文献名称**: *Production of monoclonal antibodies specific for human mitochondrial aspartate aminotransferase*
**作者**: Kamimoto Y, et al.
**摘要**: 该研究通过免疫小鼠制备了针对人线粒体AST(mAST)的单克隆抗体，验证其对mAST的特异性识别能力，并成功应用于区分细胞质AST(cAST)与线粒体AST的同工酶检测。
---
2. **文献名称**: *Aspartate aminotransferase (AST) antibody-based ELISA for detection of liver injury*
**作者**: Ozer J, et al.
**摘要**: 开发了一种基于抗AST抗体的高灵敏度ELISA检测方法，用于定量血清AST水平，评估其在药物性肝损伤模型中的应用，证明其优于传统酶活性分析法。
---
3. **文献名称**: *Immunohistochemical localization of aspartate aminotransferase in cardiac tissue*
**作者**: Smith CE, et al.
**摘要**: 利用抗AST抗体进行心脏组织免疫组化分析，揭示了AST在心肌细胞中的亚细胞定位，为研究心肌缺血后AST释放机制提供了形态学依据。
---
**注**：以上文献为示例性内容，实际引用需通过PubMed、Web of Science等数据库检索最新或经典研究，并核对作者、年份及具体实验细节。
Aspartate aminotransferase (AST), also known as glutamic oxaloacetic transaminase (GOT), is a key enzyme involved in amino acid metabolism, catalyzing the reversible transfer of an amino group between aspartate and glutamate. It exists in two isoforms: a mitochondrial form (AST1) and a cytosolic form (AST2), encoded by separate genes. AST is abundantly expressed in the liver, heart, skeletal muscle, kidneys, and brain, making it a critical biomarker for tissue damage, particularly in liver disorders (e.g., hepatitis, cirrhosis) and myocardial infarction.
AST antibodies are immunodetection tools specifically designed to recognize and bind to AST proteins in research and diagnostic applications. These antibodies are typically produced in host animals (e.g., rabbits, mice) using purified AST antigens, followed by affinity purification to ensure specificity. They enable the quantification and localization of AST in tissues or biological fluids through techniques like Western blotting, immunohistochemistry (IHC), and enzyme-linked immunosorbent assays (ELISA). In clinical diagnostics, AST antibody-based assays complement enzymatic activity measurements to confirm elevated AST levels, aiding in the differential diagnosis of organ-specific injuries. Researchers also utilize these antibodies to study AST's role in metabolic pathways, cellular stress responses, and diseases linked to mitochondrial dysfunction. Validation parameters, including cross-reactivity with isoform variants and species specificity, are critical to ensure reliability in experimental or clinical settings.
×
