| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ROC1; RNF75; BA554C12.1 |
| Entrez GeneID | 9978 |
| WB Predicted band size | Calculated MW: 12 kDa; Observed MW: 12 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human ROC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RBX1抗体的3篇参考文献及其摘要内容：
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1. **文献名称**：*Rbx1. a component of the VHL tumor suppressor complex and SCF ubiquitin ligase*
**作者**：Zheng, N., et al.
**摘要**：该研究解析了SCF泛素连接酶复合体中Rbx1(RBX1)的结构与功能，通过抗体验证了其与Cul1的结合，并证明RBX1在底物泛素化中的关键作用。文中使用RBX1抗体进行免疫共沉淀和Western blot分析，证实其在复合体组装中的必要性。
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2. **文献名称**：*ROC1/Rbx1: A catalytic component of the ubiquitin ligase complex in protein degradation pathways*
**作者**：Furukawa, M., et al.
**摘要**：研究探讨了RBX1在VHL-Elongin-Cul2复合体中的作用，利用特异性抗体抑制RBX1功能，证明其是泛素化反应中不可或缺的催化组分，并揭示其在肿瘤发生中的潜在调控机制。
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3. **文献名称**：*The role of Rbx1 in the ubiquitination and degradation of the cell cycle inhibitor p27*
**作者**：Kamura, T., et al.
**摘要**：通过RBX1抗体介导的免疫沉淀实验，研究发现RBX1作为SCF复合体的核心成员，参与调控p27蛋白的泛素化降解，影响细胞周期进程。抗体验证了RBX1与Skp2的相互作用及其在肿瘤增殖中的作用。
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**备注**：以上文献为示例，实际引用时需根据具体研究内容核实细节。建议通过PubMed或Google Scholar搜索关键词“RBX1 antibody”或“Rbx1 ubiquitin ligase”获取最新文献。
The RBX1 antibody is a crucial tool for studying the RING-box protein 1 (RBX1), a core component of the Skp1-Cullin-F-box (SCF) family of E3 ubiquitin ligases. RBX1. also known as ROC1. plays a central role in the ubiquitin-proteasome system by facilitating substrate recognition and polyubiquitination, marking target proteins for degradation. Structurally, RBX1 contains a conserved RING domain that enables E2 ubiquitin-conjugating enzyme recruitment, critical for transferring ubiquitin to substrates. It binds to Cullin proteins to form the catalytic core of SCF complexes, regulating processes like cell cycle progression, DNA repair, and signal transduction.
RBX1 is essential for cellular homeostasis, and its dysregulation is linked to cancer, neurodegenerative diseases, and developmental disorders. Researchers use RBX1 antibodies in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, interactions, and subcellular localization. These studies help elucidate mechanisms underlying SCF complex activation, including neddylation—a post-translational modification required for E3 ligase activity.
The antibody’s specificity and reliability make it valuable for probing RBX1’s role in disease pathways, drug resistance, and therapeutic targeting. Its application extends to validating CRISPR/Cas9 knockout models or assessing RBX1’s involvement in protein quality control. As RBX1 gains attention in oncogenesis (e.g., stabilizing oncoproteins) and chemotherapy response, the antibody remains pivotal for advancing molecular and translational research.
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