| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | REL |
| Entrez GeneID | 5966 |
| WB Predicted band size | Calculated MW: 69 kDa; Observed MW: 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human c-Rel |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于c-Rel抗体的代表性文献示例(注：文献信息为模拟概括，具体需通过学术数据库验证)：
1. **文献名称**：*"c-Rel is a critical mediator of NF-κB-dependent gene expression in lymphocytes"*
**作者**：Gerondakis, S., et al.
**摘要**：该研究利用c-Rel特异性抗体，通过染色质免疫沉淀(ChIP)和基因敲除模型，揭示了c-Rel在淋巴细胞中调控特定NF-κB靶基因(如IL-2和Bcl-xL)的核心作用，强调了其在免疫应答中的功能特异性。
2. **文献名称**：*"Structural basis of c-Rel activation by IκB kinase-induced phosphorylation"*
**作者**：Chen, Y., et al.
**摘要**：通过结合c-Rel抗体的免疫印迹和结构生物学技术，该文献阐明了IκB激酶(IKK)介导的c-Rel磷酸化对其核转位及DNA结合活性的调控机制，为靶向c-Rel的炎症性疾病治疗提供了理论依据。
3. **文献名称**：*"c-Rel antibody-based profiling identifies aberrant NF-κB activation in diffuse large B-cell lymphoma"*
**作者**：Davis, R.E., et al.
**摘要**：研究使用高特异性c-Rel抗体对淋巴瘤组织进行免疫组化分析，发现c-Rel在部分弥漫大B细胞淋巴瘤(DLBCL)中异常激活，并与患者预后不良相关，提示其作为潜在治疗靶点的价值。
(注：以上文献信息为示例性质，实际引用需查询PubMed、Google Scholar等平台获取真实文献。)
The c-Rel antibody is a crucial tool for studying the c-Rel protein, a member of the NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) transcription factor family. c-Rel plays a pivotal role in regulating immune and inflammatory responses by controlling the expression of genes involved in cell survival, proliferation, and cytokine production. Structurally, c-Rel contains a conserved N-terminal Rel homology domain (RHD) responsible for DNA binding, dimerization, and interaction with inhibitory IκB proteins, and a C-terminal transactivation domain (TAD) that mediates transcriptional activation.
c-Rel is primarily expressed in immune cells, including T and B lymphocytes, macrophages, and dendritic cells. Its activation is tightly regulated through canonical and non-canonical NF-κB signaling pathways, often triggered by cytokines, pathogens, or stress signals. Dysregulation of c-Rel has been implicated in autoimmune diseases (e.g., rheumatoid arthritis), chronic inflammation, and cancers (e.g., lymphomas), highlighting its dual role as both a tumor suppressor and promoter depending on cellular context.
Antibodies targeting c-Rel are widely used in techniques like Western blotting, immunohistochemistry, chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate its expression, localization, and DNA-binding activity. These antibodies help elucidate c-Rel's involvement in disease mechanisms and its potential as a therapeutic target. Researchers also utilize c-Rel inhibitors to explore pathways linked to NF-κB signaling, offering insights for drug development in inflammation and oncology.
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