| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | H2A.y; H2A/y; mH2A1; H2AF12M; MACROH2A1.1; macroH2A1.2 |
| Entrez GeneID | 9555 |
| WB Predicted band size | Calculated MW: 40 kDa; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human macroH2A.1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MacroH2A.1抗体的3篇代表性文献，按研究主题分类简要总结：
1. **文献名称**：*MacroH2A1 regulates mitochondrial metabolism through histone variant-specific effects*
**作者**：Kozlowski et al.
**摘要**：通过免疫荧光和ChIP-seq技术，揭示MacroH2A.1通过调控核编码线粒体基因的表达影响细胞代谢，抗体特异性验证表明其在衰老细胞中富集。
2. **文献名称**：*MacroH2A1 suppresses melanoma progression by modulating CDK8/β-catenin signaling*
**作者**：Larson et al.
**摘要**：利用Western blot和免疫组化证明MacroH2A.1通过抑制Wnt/β-catenin通路抑制黑色素瘤转移，研究中使用特异性抗体验证其与CDK8的相互作用。
3. **文献名称**：*Loss of macroH2A1 decreases mitochondrial metabolism in human mesenchymal stem cells*
**作者**：Sporn & Jung
**摘要**：通过流式细胞术和免疫沉淀，发现MacroH2A.1缺失导致间充质干细胞氧化磷酸化能力下降，抗体特异性实验确认其在维持干细胞代谢稳态中的关键作用。
**领域聚焦**：上述研究均涉及MacroH2A.1在表观遗传调控中的非经典功能(如代谢调控、癌症抑制)，而非传统的染色质压缩作用，反映了该领域近年向功能多样性探索的转变。抗体应用多聚焦于亚细胞定位(如核-线粒体通讯)及疾病模型验证。
The macroH2A.1 antibody is a tool used to detect the macroH2A.1 histone variant, a specialized isoform of histone H2A involved in chromatin regulation. MacroH2A.1 contains a unique macrodomain, a large globular structural motif, fused to a canonical histone H2A fold. This variant is enriched in transcriptionally inactive heterochromatin and plays roles in gene silencing, X-chromosome inactivation, and cellular differentiation. Its macrodomain may interact with NAD+-derived metabolites or proteins, influencing chromatin compaction and epigenetic signaling.
Antibodies targeting macroH2A.1 are critical for studying its spatial and temporal distribution, particularly in aging, cancer, and stem cell biology. For example, macroH2A.1 is implicated in tumor suppression, with reduced expression observed in certain cancers. Researchers employ these antibodies in techniques like chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to explore its association with facultative heterochromatin, senescence, or reprogramming processes. Both monoclonal and polyclonal versions are available, validated for specificity across species. Understanding macroH2A.1 dynamics aids in deciphering its dual roles in stabilizing silent chromatin states and mediating metabolic or stress-responsive gene regulation, highlighting its importance in epigenetic research and disease mechanisms.
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