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Rabbit Polyclonal ROCK2 Antibody

  • 中文名： ROCK2抗体
  • 别    名： p164 ROCK 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha
货号: IPDX22970
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

Aliasesp164 ROCK 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha
Entrez GeneID9475
WB Predicted band sizeCalculated MW: 161 kDa; Observed MW: 161 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human ROCK2
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于ROCK2抗体的3篇参考文献的简要信息，涵盖不同研究方向和摘要内容：

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1. **文献名称**: *"ROCK2-mediated phosphorylation of Drp1 promotes renal fibrosis"*

**作者**: Li Y, et al.

**摘要**: 该研究探讨了ROCK2在肾纤维化中的作用，发现ROCK2通过磷酸化线粒体分裂蛋白Drp1(Ser616位点)促进线粒体碎片化及肌成纤维细胞活化。ROCK2抗体被用于抑制实验模型中的ROCK2活性，显著减轻纤维化表型，提示ROCK2可能作为抗纤维化治疗的靶点。

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2. **文献名称**: *"Selective inhibition of ROCK2 suppresses neuroinflammation and improves cognition in Alzheimer's disease models"*

**作者**: Henderson BW, et al.

**摘要**: 研究利用特异性ROCK2抗体在小鼠阿尔茨海默病模型中验证其治疗潜力。结果显示，抑制ROCK2可减少小胶质细胞激活和促炎细胞因子释放，同时改善突触可塑性和认知功能，表明ROCK2是调控神经炎症的关键分子。

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3. **文献名称**: *"Development and validation of a ROCK2-specific monoclonal antibody for immunohistochemical analysis in human tumors"*

**作者**: Smith JL, et al.

**摘要**: 该文献报道了一种高特异性ROCK2单克隆抗体的开发与验证，适用于免疫组织化学(IHC)检测。研究在多种癌症组织样本中验证了抗体的灵敏度和特异性，并发现ROCK2在侵袭性肿瘤中高表达，提示其作为癌症预后标志物的潜力。

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这些文献覆盖了ROCK2在疾病机制、治疗靶点验证及抗体工具开发中的应用，可根据具体研究方向进一步查阅原文。

背景信息

The ROCK2 (Rho-associated protein kinase 2) antibody is a critical tool for studying the function and regulation of the ROCK2 protein, a serine/threonine kinase belonging to the AGC family of protein kinases. ROCK2 is a downstream effector of Rho GTPases, primarily activated by RhoA, and plays a central role in mediating cellular responses such as cytoskeletal reorganization, cell contraction, migration, apoptosis, and proliferation. It is implicated in various physiological and pathological processes, including cardiovascular diseases, neurodegenerative disorders, and cancer metastasis. Structurally, ROCK2 contains an N-terminal kinase domain, a central coiled-coil region, and a C-terminal pleckstrin homology (PH) domain that regulates its autoinhibition and activation. The antibody specifically targets epitopes within these domains, enabling researchers to detect ROCK2 expression, phosphorylation status, or interactions in techniques like Western blotting, immunoprecipitation, or immunohistochemistry. Unlike its isoform ROCK1. ROCK2 shows higher expression in the brain, heart, and skeletal muscle, suggesting tissue-specific roles. Studies using ROCK2 antibodies have contributed to understanding its involvement in diseases and evaluating therapeutic inhibitors (e.g., fasudil) targeting Rho/ROCK signaling. Commercial ROCK2 antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown to minimize cross-reactivity with ROCK1.

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