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Rabbit Polyclonal Rad50 Antibody

  • 中文名： Rad50抗体
  • 别    名： RAD50; DNA repair protein RAD50; hRAD50
货号: IPDX22966
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/500-1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 1/50-1/200 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRAD50; DNA repair protein RAD50; hRAD50
Entrez GeneID10111
WB Predicted band sizeCalculated MW: 154 kDa; Observed MW: 154 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human Rad50
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于Rad50抗体的参考文献列表(简要概括)：

1. **文献名称**：*The DNA double-strand break repair gene hMRE11 is mutated in individuals with an ataxia-telangiectasia-like disorder*

**作者**：Stewart GS et al.

**摘要**：该研究通过免疫沉淀和Western blot分析，使用Rad50抗体验证hMRE11与Rad50在DNA损伤修复中的相互作用，揭示了其在遗传性疾病中的功能缺陷。

2. **文献名称**：*NBS1 and the integration of phosphorylation-dependent signaling in the DNA damage response*

**作者**：Lee JH, Paull TT

**摘要**：利用Rad50抗体进行免疫荧光染色，研究Rad50在DNA损伤后与NBS1的共定位，阐明其在信号传导和修复灶形成中的关键作用。

3. **文献名称**：*Mre11-Rad50 complex structure and its functional implications*

**作者**：Williams GJ et al.

**摘要**：通过Rad50抗体的Western blot和免疫共沉淀技术，解析Mre11-Rad50复合物的结构，证明其ATP酶活性对DNA末端处理的调控机制。

4. **文献名称**：*Distinct roles of Rad50 and Mre11 in repairing double-strand breaks*

**作者**：Stracker TH, Petrini JH

**摘要**：研究利用Rad50抗体在小鼠模型中敲除实验，揭示Rad50在维持基因组稳定性中不可或缺的功能，尤其在非同源末端连接修复途径中的作用。

以上文献均涉及Rad50抗体的实验应用(如Western blot、免疫荧光等)，重点探讨Rad50在DNA修复机制中的分子功能。

背景信息

The Rad50 antibody is a crucial tool in studying the Rad50 protein, a key component of the Mre11-Rad50-Nbs1 (MRN) complex involved in DNA damage response and repair. Rad50. conserved across eukaryotes, functions as a DNA-binding ATPase that coordinates double-strand break (DSB) repair via homologous recombination, non-homologous end joining, and meiotic recombination. Its structural role includes bridging DNA ends and activating checkpoint signaling. Researchers use Rad50 antibodies to detect protein expression, localization, and interactions in techniques like Western blotting, immunofluorescence, co-immunoprecipitation, and chromatin immunoprecipitation. These antibodies help evaluate Rad50's involvement in maintaining genomic stability, telomere regulation, and meiosis. Dysfunctional Rad50 is linked to cancer predisposition, immunodeficiency, and radiation sensitivity. Commercial Rad50 antibodies are typically raised in rabbits or mice, validated for specificity against human, mouse, or other model organisms. Recent studies focus on Rad50's role in chemotherapy/radiotherapy resistance and its potential as a predictive biomarker. Proper controls, including knockout cell lines, are essential to confirm antibody specificity due to possible cross-reactivity with structurally similar proteins.

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