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产品描述：人αII-血影蛋白分解产物SBDP145酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、脑脊液（CSF）样本中的SBDP145含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，在科研领域，广泛应用于创伤性脑损伤模型评估、神经退行性疾病机制研究、细胞凋亡信号通路分析等方向，尤其适用于脑脊液样本中神经损伤标志物的动态监测，以及通过组织匀浆分析局部损伤程度，为神经病理学、药理学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：SBDP145
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cerebrospinal fluid (CSF)
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human SBDP145 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 105 | ||||
| Range % | 100-109 | |||||
回收率：
| The recovery of human SBDP145 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 85-90 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 402.5522.6782.6152.447 202.0492.0862.0681.900 101.4581.4691.4641.296 50.9120.9540.9330.765 2.50.6360.6780.6570.489 1.250.4420.4680.4550.287 0.6250.3410.3510.3460.178 00.1670.1680.168 | ||||||
货期：3-5 working days
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