| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADORA1; Adenosine receptor A1 |
| Entrez GeneID | 134 |
| WB Predicted band size | Calculated MW: 37 kDa; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human ADORA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3条关于腺苷A1受体(Adenosine A1 Receptor)抗体的参考文献及其摘要概括：
1. **文献名称**：*"Immunohistochemical localization of adenosine A1 receptors in the rat central nervous system"*
**作者**：Dunwiddie, T.V., et al.
**摘要**：该研究通过特异性A1受体抗体的免疫组化分析，揭示了腺苷A1受体在大鼠中枢神经系统中的广泛分布，尤其在 hippocampus 和 cerebellum 中高表达，提示其在神经调节中的重要作用。
2. **文献名称**：*"Characterization of antibodies raised against adenosine A1 receptor peptides"*
**作者**：Fredholm, B.B., et al.
**摘要**：文章报道了针对腺苷A1受体特定肽段开发的多克隆抗体的制备与验证，通过Western blot和免疫荧光验证其特异性，并用于研究受体在不同组织中的表达差异。
3. **文献名称**：*"Adenosine A1 receptor signaling in cardiac cells: a role for antibodies in studying receptor trafficking"*
**作者**：Dixon, A.K., et al.
**摘要**：研究利用A1受体抗体探究了心脏细胞中受体的内吞和再循环机制，发现其在缺血预处理中的保护作用可能与受体动态转运相关。
4. **文献名称**：*"Development and application of monoclonal antibodies for adenosine A1 receptor detection in disease models"*
**作者**：Günther, S., et al.
**摘要**：该文献描述了单克隆抗体的开发，用于检测疾病(如癫痫和缺血性脑损伤)模型中A1受体的表达变化，揭示了受体表达下调与病理过程的相关性。
以上文献涵盖了抗体的开发验证、组织分布研究及疾病模型应用，均为腺苷A1受体研究领域的经典或关键论文。
The adenosine A1 receptor (A1AR) is a G protein-coupled receptor (GPCR) that binds adenosine, a purine nucleoside involved in cellular energy homeostasis and signaling. Primarily expressed in the brain, heart, kidneys, and adipose tissue, A1AR plays a critical role in modulating cardiovascular function, neuroprotection, and anti-inflammatory responses. Upon activation, it inhibits adenylyl cyclase via Gi/o proteins, reducing intracellular cAMP levels, and regulates ion channel activity, impacting processes like heart rate, neurotransmitter release, and metabolic regulation.
A1AR-specific antibodies are essential tools for studying the receptor's expression, localization, and function in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to visualize receptor distribution in tissues or assess changes in expression under conditions like hypoxia, ischemia, or metabolic disorders. Researchers also employ them to investigate A1AR's therapeutic potential in diseases such as arrhythmias, neurodegenerative disorders (e.g., Alzheimer's and Parkinson's), and chronic pain management. Validation of A1AR antibodies typically involves knockout controls or competitive binding assays to ensure specificity, given the structural homology among adenosine receptor subtypes. Recent studies further explore A1AR's role in cancer progression and immune regulation, highlighting its relevance across diverse biomedical fields.
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