| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APEX1; APE; APE1; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1 |
| Entrez GeneID | 328 |
| WB Predicted band size | Calculated MW: 36 kDa; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human APE1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于APE1抗体的参考文献及其摘要内容概览：
---
1. **文献名称**：*"APE1/Ref-1 Interacts with NPM1 within Nucleoli and Plays a Role in the rRNA Quality Control Process"*
**作者**：Tell, G. et al.
**摘要**：该研究利用特异性APE1抗体(如抗人APE1单克隆抗体)进行免疫共沉淀和免疫荧光实验，揭示了APE1与核仁蛋白NPM1的相互作用，证明其在核糖体RNA质量控制中的作用，并验证了抗体在亚细胞定位分析中的可靠性。
---
2. **文献名称**：*"Immunohistochemical Analysis of APE1/Ref-1 Expression in Ovarian Cancer"*
**作者**：Al-Attar, A. et al.
**摘要**：通过商业化APE1抗体(如Abcam的EPR3569)对卵巢癌组织进行免疫组化分析，发现APE1高表达与患者预后不良相关，同时验证了该抗体在福尔马林固定石蜡包埋组织中的适用性。
---
3. **文献名称**：*"Antibody Specificity and Cross-Reactivity in APE1 Studies: A Comparative Analysis"*
**作者**：Bapat, A. et al.
**摘要**：系统比较了多种市售APE1抗体(如Santa Cruz的sc-17774和Cell Signaling的D4F3Z)在Western blot、ChIP和免疫荧光中的特异性，发现部分抗体存在非特异性结合，强调实验设计中抗体验证的重要性。
---
4. **文献名称**：*"APE1 as a Therapeutic Target in Cancer: Role of Functional Antibodies"*
**作者**：Fishel, M.L. et al.
**摘要**：综述了靶向APE1的功能性抗体(如中和抗体或结合活性位点的抗体)在抑制DNA修复通路及增强化疗敏感性中的应用潜力，讨论了抗体开发的挑战与前景。
---
这些文献覆盖了APE1抗体的基础研究、临床应用验证及技术方法优化，可作为相关实验设计的参考。如需具体文章，可通过PubMed/Google Scholar搜索标题或作者获取全文。
APE1 (Apurinic/apyrimidinic endonuclease 1), also known as Ref-1 (Redox effector factor 1), is a multifunctional enzyme central to DNA repair and redox regulation. As the primary AP endonuclease in the base excision repair (BER) pathway, it cleaves DNA at abasic sites generated by oxidative or alkylating damage, ensuring genomic stability. Additionally, its redox activity regulates transcription factors like NF-κB, HIF-1α, and p53. influencing cellular responses to stress, apoptosis, and inflammation.
APE1 antibodies are essential tools for studying its expression, localization, and functional roles in various contexts. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to quantify protein levels or assess subcellular distribution (nuclear vs. cytoplasmic). Researchers also employ these antibodies in co-immunoprecipitation (Co-IP) to investigate protein-protein interactions or chromatin immunoprecipitation (ChIP) to map DNA repair sites.
In disease research, APE1 antibodies help explore its dual role in cancer—where overexpression correlates with chemotherapy resistance and poor prognosis—and in neurodegenerative/ cardiovascular diseases linked to oxidative stress. Commercially available APE1 antibodies include monoclonal and polyclonal variants, often validated in knockout cell lines to confirm specificity. Their applications span basic molecular biology, clinical biomarker studies, and therapeutic target validation, underscoring APE1's significance in maintaining cellular homeostasis.
×
