| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HMG 2; HMG B2; HMG-2; HMG2; HMGB2 |
| Entrez GeneID | 3148 |
| WB Predicted band size | Calculated MW: 24 kDa; Observed MW: 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human HMGB2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与HMGB2抗体相关的虚拟参考文献示例(注：文献为虚构，仅用于格式参考)：
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1. **文献名称**: HMGB2 expression in colorectal cancer and its clinical significance
**作者**: Zhang L, et al.
**摘要**: 通过免疫组化(使用HMGB2单克隆抗体)分析结直肠癌组织中HMGB2蛋白表达水平，发现其高表达与肿瘤转移和不良预后显著相关，提示HMGB2可能作为潜在治疗靶点。
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2. **文献名称**: HMGB2 regulates embryonic stem cell differentiation via chromatin remodeling
**作者**: Tanaka S, et al.
**摘要**: 利用HMGB2特异性抗体进行ChIP-seq和Western blot实验，证明HMGB2通过调控染色质可及性影响胚胎干细胞向中胚层分化的过程，敲低HMGB2导致分化关键基因沉默。
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3. **文献名称**: Development of a novel HMGB2 polyclonal antibody for detecting inflammatory responses
**作者**: Wang Y, et al.
**摘要**: 报道一种新型兔源HMGB2多克隆抗体的制备与验证，该抗体在ELISA和免疫荧光中显示高特异性，成功应用于脓毒症患者血清中HMGB2的检测，揭示其与炎症严重程度正相关。
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4. **文献名称**: HMGB2 mediates drug resistance in glioblastoma through autophagy regulation
**作者**: Chen H, et al.
**摘要**: 使用HMGB2中和抗体阻断其功能后，胶质母细胞瘤细胞对替莫唑胺的敏感性显著提高，机制研究表明HMGB2通过激活自噬途径促进肿瘤细胞存活。
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(提示：实际文献需通过PubMed、Google Scholar等平台以“HMGB2 antibody”或“HMGB2 function”为关键词检索。)
The High Mobility Group Box 2 (HMGB2) antibody is a tool used to detect and study the HMGB2 protein, a member of the HMG-box DNA-binding protein family. HMGB2 contains two conserved HMG domains that enable it to bend DNA and regulate chromatin architecture, facilitating processes like transcription, replication, and DNA repair. Unlike its widely studied homolog HMGB1. HMGB2 exhibits more restricted expression patterns, predominantly in germ cells, certain stem cells, and malignant tissues. It plays roles in cellular proliferation, differentiation, and maintaining pluripotency in stem cells.
Research using HMGB2 antibodies has linked this protein to cancer progression, where its overexpression correlates with tumorigenesis, metastasis, and poor prognosis. It is also implicated in developmental processes and reproductive biology. Antibodies against HMGB2 (polyclonal or monoclonal) are employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze its expression, localization, and interactions. Some studies explore HMGB2's extracellular signaling roles in inflammation, though this is less characterized than HMGB1. Epitope-tagged HMGB2 antibodies (e.g., FLAG or HA) further aid in tracking recombinant protein expression. Recent interest focuses on developing HMGB2-targeted therapies, particularly in oncology, leveraging its selective expression in malignancies.
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