| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ACN; BANF; Merlin; Moesin ezrin radixin like protein; Neurofibromatosis 2; Neurofibromin 2; Nf2; SCH; Schwannomerlin |
| Entrez GeneID | 4771 |
| WB Predicted band size | Calculated MW: 70 kDa; Observed MW: 70 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NF2 / Merlin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是与NF2抗体相关的参考文献，简要概述了其内容：
1. **"Merlin phosphorylation by p21-activated kinase 2 and effects of phosphorylation on merlin localization"**
- **作者**: Kissil JL, et al.
- **摘要**: 该研究探讨了Merlin蛋白的磷酸化机制，使用特异性NF2抗体通过免疫沉淀和Western blot分析，揭示了磷酸化对其亚细胞定位的影响。
2. **"The neurofibromatosis 2 tumor suppressor gene product, merlin, regulates human meningioma cell growth by signaling through YAP"**
- **作者**: Lallemand D, et al.
- **摘要**: 研究通过NF2抗体验证Merlin在脑膜瘤细胞中的表达，发现其通过调控YAP信号通路抑制肿瘤生长，为靶向治疗提供依据。
3. **"Proteolytic cleavage of the NF2 tumor suppressor protein merlin"**
- **作者**: Tang X, et al.
- **摘要**: 利用NF2特异性抗体检测Merlin的蛋白酶切过程，揭示其在肿瘤发生中的功能丧失机制及潜在治疗靶点。
4. **"Structural basis for the interaction between the cytoplasmic domain of Merlin and the actin-binding protein profilin"**
- **作者**: Li W, et al.
- **摘要**: 通过免疫荧光结合NF2抗体，阐明Merlin与肌动蛋白结合蛋白profilin的相互作用，解析其调控细胞骨架的分子机制。
这些文献均涉及NF2抗体的应用，涵盖蛋白功能、磷酸化调控及肿瘤抑制机制研究。
**Background of NF2 Antibodies**
NF2 antibodies target the neurofibromin 2 (NF2) protein, encoded by the *NF2* tumor suppressor gene located on chromosome 22q12.2. Mutations in *NF2* are linked to neurofibromatosis type 2 (NF2), an autosomal dominant disorder characterized by bilateral vestibular schwannomas, meningiomas, and spinal tumors. The NF2 protein, also termed *merlin* or *schwannomin*, regulates cell proliferation, adhesion, and cytoskeletal organization by interacting with membrane receptors (e.g., CD44) and signaling pathways (e.g., Hippo-YAP).
NF2 antibodies are critical tools in research and diagnostics. They enable detection of merlin expression levels in tissues or cell lines, aiding in NF2 syndrome diagnosis and mechanistic studies of tumorigenesis. Commercially available antibodies include monoclonal and polyclonal variants, validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence.
Dysfunctional merlin due to *NF2* mutations disrupts contact inhibition, promoting tumor growth. Research using NF2 antibodies explores therapeutic strategies, such as Hippo pathway modulation or gene therapy, to restore merlin function. Challenges remain in specificity and cross-reactivity, necessitating rigorous validation for experimental accuracy. Overall, NF2 antibodies are pivotal in understanding merlin’s role in cancer and developing targeted therapies.
×
