| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ATG4C; APG4C; AUTL1; AUTL3; Cysteine protease ATG4C; AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C |
| Entrez GeneID | 84938 |
| WB Predicted band size | Calculated MW: 52 kDa; Observed MW: 52 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Atg4C |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ATG4C抗体的3篇文献示例(文献信息为模拟内容，供参考)：
1. **文献名称**: "ATG4C regulates autophagy and apoptosis in cancer cells via LC3 processing"
**作者**: Marino G, et al.
**摘要**: 研究揭示了ATG4C通过调控LC3蛋白的切割过程参与自噬体形成，并发现其表达水平在结肠癌中异常下调，与肿瘤抑制相关。使用特异性ATG4C抗体证实了其在癌细胞中的定位及功能缺失对自噬-凋亡平衡的影响。
2. **文献名称**: "The role of ATG4C in metabolic stress adaptation revealed by knockout mouse models"
**作者**: Sou YS, et al.
**摘要**: 通过构建ATG4C基因敲除小鼠模型，结合抗体免疫组化分析，发现ATG4C缺失导致肝脏和肌肉组织中自噬功能受损，加剧高脂饮食诱导的代谢紊乱，提示其在代谢疾病中的潜在治疗价值。
3. **文献名称**: "ATG4C as a biomarker for neurodegenerative disease progression"
**作者**: Li M, et al.
**摘要**: 该研究利用ATG4C特异性抗体检测阿尔茨海默病模型小鼠脑组织，发现ATG4C蛋白表达水平与tau蛋白异常聚集呈负相关，提示其可能作为神经退行性疾病的生物标志物或干预靶点。
(注：以上文献为示例，实际引用需根据具体研究通过PubMed或学术数据库检索核实。)
ATG4C, a member of the ATG4 cysteine protease family, plays a critical role in autophagy, a cellular recycling process essential for maintaining homeostasis. It specifically processes LC3 (microtubule-associated protein 1A/1B-light chain 3), a key autophagy-related protein, by cleaving its C-terminal region to generate cytosolic LC3-I. This step is crucial for LC3-I’s subsequent lipidation to LC3-II, which integrates into autophagosomal membranes. Additionally, ATG4C mediates the delipidation of LC3-II during autophagosome-lysosome fusion, enabling LC3 recycling. Dysregulation of ATG4C has been linked to cancer, neurodegenerative disorders, and metabolic diseases, making it a focus in autophagy-related pathology studies.
ATG4C antibodies are vital tools for detecting and quantifying ATG4C expression, localization, and activity in research. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study autophagy dynamics under physiological or stress conditions. Polyclonal and monoclonal variants offer flexibility, with some antibodies specifically targeting post-translational modifications (e.g., phosphorylation) to explore regulatory mechanisms. These antibodies help elucidate ATG4C’s interactions with substrates like LC3 and its functional crosstalk with other ATG4 homologs (ATG4A/B/D). As autophagy gains attention in therapeutic research, ATG4C antibodies also support drug discovery efforts aiming to modulate autophagy in diseases such as cancer or neurodegeneration.
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