| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 1/20 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/50-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 2.7.1.21; KITH; TK-1; TK1; Thymidine kinase 1; cytosolic |
| Entrez GeneID | 7083 |
| WB Predicted band size | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Thymidine Kinase 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Thymidine Kinase抗体的3篇参考文献及其摘要概括：
1. **"Thymidine kinase 1 as a biomarker for cancer prognosis: Immunoassays and clinical utility"**
*作者：H. Hagberg, E. Gustavsson*
**摘要**：探讨了Thymidine Kinase 1(TK1)抗体在癌症预后评估中的应用，开发了高灵敏度的ELISA检测方法，证明血清TK1水平与肿瘤增殖活性相关，可用于乳腺癌和淋巴瘤的早期监测。
2. **"Development of monoclonal antibodies against human thymidine kinase 1 for cancer diagnostics"**
*作者：J. Zhang et al.*
**摘要**：研究团队制备了针对人TK1的单克隆抗体，验证其在免疫组化和血清学检测中的特异性，表明其可作为结直肠癌和肺癌的潜在生物标志物。
3. **"Herpes simplex virus thymidine kinase antibodies in herpes encephalitis diagnosis"**
*作者：R. L. Burke et al.*
**摘要**：开发了针对HSV Thymidine Kinase的抗体检测方法，用于区分单纯疱疹病毒(HSV)感染与其他脑炎病因，提升了诊断特异性与早期干预效果。
如有其他需求可进一步补充调整。
Thymidine kinase (TK) antibodies are immunological tools used to detect and quantify thymidine kinase enzymes, which play critical roles in DNA synthesis and cellular proliferation. Thymidine kinases exist in two primary forms: TK1 and TK2. TK1. a cytosolic enzyme, is involved in the salvage pathway of nucleotide synthesis, converting thymidine to thymidine monophosphate during the cell cycle’s S-phase. It is highly expressed in proliferating cells and is often elevated in malignancies, making it a potential biomarker for cancer monitoring. TK2. located in mitochondria, is essential for mitochondrial DNA synthesis and is associated with genetic disorders like TK2-deficient mitochondrial DNA depletion syndrome.
Antibodies targeting TK1 or TK2 are widely utilized in research and diagnostics. TK1-specific antibodies, for instance, help assess cell proliferation rates in cancers, evaluate treatment responses, or predict relapse risk. Commercial TK1 ELISA kits, relying on these antibodies, measure serum TK1 levels as a non-invasive cancer screening tool. TK2 antibodies are employed in studying mitochondrial diseases, aiding in diagnosis or mechanistic research. Both polyclonal and monoclonal TK antibodies are used in techniques like Western blotting, immunohistochemistry, and flow cytometry to localize or quantify enzyme expression.
Research on TK antibodies also explores their therapeutic potential, such as antibody-drug conjugates targeting TK-overexpressing cancer cells. However, challenges remain in ensuring specificity, minimizing cross-reactivity, and standardizing assays for clinical use. Overall, TK antibodies serve as vital reagents bridging basic research, diagnostics, and therapeutic development in oncology and genetic disorders.
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